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Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG

Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter found in the central nervous system of mammals. A range of bacterial species can synthesize GABA, including Lactobacillus plantarum of which L-monosodium glutamate (L-MSG) is an inducer of its production. In order to synthesize GABA in...

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Autores principales: Zhuang, Kejin, Jiang, Yujun, Feng, Xiaohan, Li, Li, Dang, Fangfang, Zhang, Wei, Man, Chaoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997328/
https://www.ncbi.nlm.nih.gov/pubmed/29894506
http://dx.doi.org/10.1371/journal.pone.0199021
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author Zhuang, Kejin
Jiang, Yujun
Feng, Xiaohan
Li, Li
Dang, Fangfang
Zhang, Wei
Man, Chaoxin
author_facet Zhuang, Kejin
Jiang, Yujun
Feng, Xiaohan
Li, Li
Dang, Fangfang
Zhang, Wei
Man, Chaoxin
author_sort Zhuang, Kejin
collection PubMed
description Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter found in the central nervous system of mammals. A range of bacterial species can synthesize GABA, including Lactobacillus plantarum of which L-monosodium glutamate (L-MSG) is an inducer of its production. In order to synthesize GABA in high concentrations, L-MSG was utilized as the single inducing factor, a chemically defined medium (CDM) was used as the fermentation substrate, with L. plantarum CGMCC 1.2437(T) cultured in medium supplemented with or without L-MSG. High-throughput transcriptome sequencing was used to explore the differential genes expression of bacterial cells at 36 h of fermentation, where the GABA concentration of CDM with L-MSG reached the peak value and was 7.7 times higher than that of medium without L-MSG at the same timepoint. A total of 87 genes showed significant differential expression induced by L-MSG: of these, 69 were up-regulated genes and 18 were down-regulated. The up-regulated genes were assigned to biological processes and molecular function, while the down-regulated genes covered biological process, cellular process and molecular function. Interrogation of results using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, indicated carbohydrate metabolism, fatty acid synthesis and amino acid metabolism were closely associated with GABA synthesis induced by L-MSG. This study provides insights into L. plantarum-mediated GABA fermentation at the molecular level and will provide a new approach for further studies related to GABA production by the other Lactic acid bacteria.
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spelling pubmed-59973282018-06-21 Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG Zhuang, Kejin Jiang, Yujun Feng, Xiaohan Li, Li Dang, Fangfang Zhang, Wei Man, Chaoxin PLoS One Research Article Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter found in the central nervous system of mammals. A range of bacterial species can synthesize GABA, including Lactobacillus plantarum of which L-monosodium glutamate (L-MSG) is an inducer of its production. In order to synthesize GABA in high concentrations, L-MSG was utilized as the single inducing factor, a chemically defined medium (CDM) was used as the fermentation substrate, with L. plantarum CGMCC 1.2437(T) cultured in medium supplemented with or without L-MSG. High-throughput transcriptome sequencing was used to explore the differential genes expression of bacterial cells at 36 h of fermentation, where the GABA concentration of CDM with L-MSG reached the peak value and was 7.7 times higher than that of medium without L-MSG at the same timepoint. A total of 87 genes showed significant differential expression induced by L-MSG: of these, 69 were up-regulated genes and 18 were down-regulated. The up-regulated genes were assigned to biological processes and molecular function, while the down-regulated genes covered biological process, cellular process and molecular function. Interrogation of results using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, indicated carbohydrate metabolism, fatty acid synthesis and amino acid metabolism were closely associated with GABA synthesis induced by L-MSG. This study provides insights into L. plantarum-mediated GABA fermentation at the molecular level and will provide a new approach for further studies related to GABA production by the other Lactic acid bacteria. Public Library of Science 2018-06-12 /pmc/articles/PMC5997328/ /pubmed/29894506 http://dx.doi.org/10.1371/journal.pone.0199021 Text en © 2018 Zhuang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhuang, Kejin
Jiang, Yujun
Feng, Xiaohan
Li, Li
Dang, Fangfang
Zhang, Wei
Man, Chaoxin
Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title_full Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title_fullStr Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title_full_unstemmed Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title_short Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437(T) induced by L-MSG
title_sort transcriptomic response to gaba-producing lactobacillus plantarum cgmcc 1.2437(t) induced by l-msg
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997328/
https://www.ncbi.nlm.nih.gov/pubmed/29894506
http://dx.doi.org/10.1371/journal.pone.0199021
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