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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined adva...

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Detalles Bibliográficos
Autores principales: Smerkova, Kristyna, Vlcnovska, Marcela, Dostalova, Simona, Milosavljevic, Vedran, Kopel, Pavel, Vaculovic, Tomas, Krizkova, Sona, Vaculovicova, Marketa, Adam, Vojtech, Kizek, Rene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997378/
https://www.ncbi.nlm.nih.gov/pubmed/29942374
http://dx.doi.org/10.5772/61699
Descripción
Sumario:Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.