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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined adva...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997378/ https://www.ncbi.nlm.nih.gov/pubmed/29942374 http://dx.doi.org/10.5772/61699 |
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author | Smerkova, Kristyna Vlcnovska, Marcela Dostalova, Simona Milosavljevic, Vedran Kopel, Pavel Vaculovic, Tomas Krizkova, Sona Vaculovicova, Marketa Adam, Vojtech Kizek, Rene |
author_facet | Smerkova, Kristyna Vlcnovska, Marcela Dostalova, Simona Milosavljevic, Vedran Kopel, Pavel Vaculovic, Tomas Krizkova, Sona Vaculovicova, Marketa Adam, Vojtech Kizek, Rene |
author_sort | Smerkova, Kristyna |
collection | PubMed |
description | Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized. |
format | Online Article Text |
id | pubmed-5997378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-59973782018-06-25 ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation Smerkova, Kristyna Vlcnovska, Marcela Dostalova, Simona Milosavljevic, Vedran Kopel, Pavel Vaculovic, Tomas Krizkova, Sona Vaculovicova, Marketa Adam, Vojtech Kizek, Rene Nanobiomedicine (Rij) Original Research Article Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized. SAGE Publications 2015-01-01 /pmc/articles/PMC5997378/ /pubmed/29942374 http://dx.doi.org/10.5772/61699 Text en © 2015 Author(s). Licensee InTech. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Smerkova, Kristyna Vlcnovska, Marcela Dostalova, Simona Milosavljevic, Vedran Kopel, Pavel Vaculovic, Tomas Krizkova, Sona Vaculovicova, Marketa Adam, Vojtech Kizek, Rene ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title | ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title_full | ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title_fullStr | ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title_full_unstemmed | ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title_short | ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation |
title_sort | elisa-like analysis of cisplatinated dna using magnetic separation |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997378/ https://www.ncbi.nlm.nih.gov/pubmed/29942374 http://dx.doi.org/10.5772/61699 |
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