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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined adva...

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Autores principales: Smerkova, Kristyna, Vlcnovska, Marcela, Dostalova, Simona, Milosavljevic, Vedran, Kopel, Pavel, Vaculovic, Tomas, Krizkova, Sona, Vaculovicova, Marketa, Adam, Vojtech, Kizek, Rene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997378/
https://www.ncbi.nlm.nih.gov/pubmed/29942374
http://dx.doi.org/10.5772/61699
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author Smerkova, Kristyna
Vlcnovska, Marcela
Dostalova, Simona
Milosavljevic, Vedran
Kopel, Pavel
Vaculovic, Tomas
Krizkova, Sona
Vaculovicova, Marketa
Adam, Vojtech
Kizek, Rene
author_facet Smerkova, Kristyna
Vlcnovska, Marcela
Dostalova, Simona
Milosavljevic, Vedran
Kopel, Pavel
Vaculovic, Tomas
Krizkova, Sona
Vaculovicova, Marketa
Adam, Vojtech
Kizek, Rene
author_sort Smerkova, Kristyna
collection PubMed
description Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.
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spelling pubmed-59973782018-06-25 ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation Smerkova, Kristyna Vlcnovska, Marcela Dostalova, Simona Milosavljevic, Vedran Kopel, Pavel Vaculovic, Tomas Krizkova, Sona Vaculovicova, Marketa Adam, Vojtech Kizek, Rene Nanobiomedicine (Rij) Original Research Article Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized. SAGE Publications 2015-01-01 /pmc/articles/PMC5997378/ /pubmed/29942374 http://dx.doi.org/10.5772/61699 Text en © 2015 Author(s). Licensee InTech. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Smerkova, Kristyna
Vlcnovska, Marcela
Dostalova, Simona
Milosavljevic, Vedran
Kopel, Pavel
Vaculovic, Tomas
Krizkova, Sona
Vaculovicova, Marketa
Adam, Vojtech
Kizek, Rene
ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title_full ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title_fullStr ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title_full_unstemmed ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title_short ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
title_sort elisa-like analysis of cisplatinated dna using magnetic separation
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997378/
https://www.ncbi.nlm.nih.gov/pubmed/29942374
http://dx.doi.org/10.5772/61699
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