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Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities

Phycoerythrin is the main phycobiliprotein that responsible for harvesting light for photosynthesis in cyanobacteria. In this research, phycoerythrin extracted from the cyanobacterium Nostoc carneum has been used to reduce silver nitrate for silver nanoparticles (AgNPs) biosynthesis. UV–visible spec...

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Autores principales: El-Naggar, Noura El-Ahmady, Hussein, Mervat H., El-Sawah, Asmaa Atallah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997762/
https://www.ncbi.nlm.nih.gov/pubmed/29895869
http://dx.doi.org/10.1038/s41598-018-27276-6
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author El-Naggar, Noura El-Ahmady
Hussein, Mervat H.
El-Sawah, Asmaa Atallah
author_facet El-Naggar, Noura El-Ahmady
Hussein, Mervat H.
El-Sawah, Asmaa Atallah
author_sort El-Naggar, Noura El-Ahmady
collection PubMed
description Phycoerythrin is the main phycobiliprotein that responsible for harvesting light for photosynthesis in cyanobacteria. In this research, phycoerythrin extracted from the cyanobacterium Nostoc carneum has been used to reduce silver nitrate for silver nanoparticles (AgNPs) biosynthesis. UV–visible spectrophotometry for measuring surface plasmon resonance showed a single absorption peak at 430 nm, which confirmed the presence of AgNPs. The face-centered central composite design was chosen to evaluate the interaction effects between four process variables and also to determine their optimal levels which influence the AgNPs biosynthesis using phycoerythrin. The maximum silver nanoparticles biosynthesis (1733.260 ± 21 µg/mL) was achieved in the central runs under the conditions of initial pH 10, incubation period of the 24 h, phycoerythrin concentration of the 0.8 mg/mL and 20 mM of AgNO(3). The biosynthesized AgNPs were characterized using TEM which revealed the formation of spherical shape nanoparticles with size ranged between 7.1‒26.68 nm. EDX analysis confirmed silver as the major constituent element. FTIR spectrum indicates the presence of proteinaceous capping agent that prevents silver nanoparticles agglomeration. The IC(50) of cell inhibition by AgNPs was observed at 13.07 ± 1.1 µg/mL. Treatment of mice bearing Ehrlich ascites carcinoma with 5 mg AgNPs/kg of mice body weight significantly decreased tumor volume, tumor cells count, white blood cells count and body weight. It was concluded that the phycoerythrin protein has the ability to synthesize AgNPs, which have antibacterial, antihemolytic, in vitro and in vivo cytotoxic activities.
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spelling pubmed-59977622018-06-21 Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities El-Naggar, Noura El-Ahmady Hussein, Mervat H. El-Sawah, Asmaa Atallah Sci Rep Article Phycoerythrin is the main phycobiliprotein that responsible for harvesting light for photosynthesis in cyanobacteria. In this research, phycoerythrin extracted from the cyanobacterium Nostoc carneum has been used to reduce silver nitrate for silver nanoparticles (AgNPs) biosynthesis. UV–visible spectrophotometry for measuring surface plasmon resonance showed a single absorption peak at 430 nm, which confirmed the presence of AgNPs. The face-centered central composite design was chosen to evaluate the interaction effects between four process variables and also to determine their optimal levels which influence the AgNPs biosynthesis using phycoerythrin. The maximum silver nanoparticles biosynthesis (1733.260 ± 21 µg/mL) was achieved in the central runs under the conditions of initial pH 10, incubation period of the 24 h, phycoerythrin concentration of the 0.8 mg/mL and 20 mM of AgNO(3). The biosynthesized AgNPs were characterized using TEM which revealed the formation of spherical shape nanoparticles with size ranged between 7.1‒26.68 nm. EDX analysis confirmed silver as the major constituent element. FTIR spectrum indicates the presence of proteinaceous capping agent that prevents silver nanoparticles agglomeration. The IC(50) of cell inhibition by AgNPs was observed at 13.07 ± 1.1 µg/mL. Treatment of mice bearing Ehrlich ascites carcinoma with 5 mg AgNPs/kg of mice body weight significantly decreased tumor volume, tumor cells count, white blood cells count and body weight. It was concluded that the phycoerythrin protein has the ability to synthesize AgNPs, which have antibacterial, antihemolytic, in vitro and in vivo cytotoxic activities. Nature Publishing Group UK 2018-06-12 /pmc/articles/PMC5997762/ /pubmed/29895869 http://dx.doi.org/10.1038/s41598-018-27276-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
El-Naggar, Noura El-Ahmady
Hussein, Mervat H.
El-Sawah, Asmaa Atallah
Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title_full Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title_fullStr Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title_full_unstemmed Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title_short Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
title_sort phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997762/
https://www.ncbi.nlm.nih.gov/pubmed/29895869
http://dx.doi.org/10.1038/s41598-018-27276-6
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