Cargando…
Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues
INTRODUCTION: Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar. METHODS & MATERIALS: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulol...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Open
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997855/ https://www.ncbi.nlm.nih.gov/pubmed/29997700 http://dx.doi.org/10.2174/1874285801812010154 |
_version_ | 1783331128836358144 |
---|---|
author | Latt, Zaw K. Yu, San S. Kyaw, Ei P. Lynn, Tin M. Nwe, May T. Mon, Wai W. Aye, Kyaw N. |
author_facet | Latt, Zaw K. Yu, San S. Kyaw, Ei P. Lynn, Tin M. Nwe, May T. Mon, Wai W. Aye, Kyaw N. |
author_sort | Latt, Zaw K. |
collection | PubMed |
description | INTRODUCTION: Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar. METHODS & MATERIALS: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS). RESULTS & DISCUSSION: The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity of A. agilis was highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity of A. agilis for cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity of A. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust. CONCLUSION: So, the isolated A. agilis has high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues. |
format | Online Article Text |
id | pubmed-5997855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Bentham Open |
record_format | MEDLINE/PubMed |
spelling | pubmed-59978552018-07-11 Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues Latt, Zaw K. Yu, San S. Kyaw, Ei P. Lynn, Tin M. Nwe, May T. Mon, Wai W. Aye, Kyaw N. Open Microbiol J Microbiology INTRODUCTION: Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar. METHODS & MATERIALS: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS). RESULTS & DISCUSSION: The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity of A. agilis was highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity of A. agilis for cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity of A. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust. CONCLUSION: So, the isolated A. agilis has high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues. Bentham Open 2018-05-31 /pmc/articles/PMC5997855/ /pubmed/29997700 http://dx.doi.org/10.2174/1874285801812010154 Text en © 2018 Latt et al. https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Microbiology Latt, Zaw K. Yu, San S. Kyaw, Ei P. Lynn, Tin M. Nwe, May T. Mon, Wai W. Aye, Kyaw N. Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title | Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title_full | Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title_fullStr | Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title_full_unstemmed | Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title_short | Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues |
title_sort | using cellulolytic nitrogen fixing bacterium, azomonas agilis for effective degradation of agricultural residues |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997855/ https://www.ncbi.nlm.nih.gov/pubmed/29997700 http://dx.doi.org/10.2174/1874285801812010154 |
work_keys_str_mv | AT lattzawk usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT yusans usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT kyaweip usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT lynntinm usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT nwemayt usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT monwaiw usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues AT ayekyawn usingcellulolyticnitrogenfixingbacteriumazomonasagilisforeffectivedegradationofagriculturalresidues |