Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice

In order to investigate the function of monkey 20α-hydroxysteroid dehydrogenase (20α-HSD), transgenic mice (tg) were produced, expressing enhanced green fluorescent protein (EGFP) under the control of the monkey 20α-HSD promoter. The expression levels and localization of EGFP and 20α-HSD were analyz...

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Autores principales: Yoo, Mi-Hyang, Park, Chae-Won, Byambaragchaa, Munkhzaya, Kang, Myung-Hwa, Min, Kwan-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Cell biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997899/
https://www.ncbi.nlm.nih.gov/pubmed/29900362
http://dx.doi.org/10.1016/j.dib.2018.05.069
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author Yoo, Mi-Hyang
Park, Chae-Won
Byambaragchaa, Munkhzaya
Kang, Myung-Hwa
Min, Kwan-Sik
author_facet Yoo, Mi-Hyang
Park, Chae-Won
Byambaragchaa, Munkhzaya
Kang, Myung-Hwa
Min, Kwan-Sik
author_sort Yoo, Mi-Hyang
collection PubMed
description In order to investigate the function of monkey 20α-hydroxysteroid dehydrogenase (20α-HSD), transgenic mice (tg) were produced, expressing enhanced green fluorescent protein (EGFP) under the control of the monkey 20α-HSD promoter. The expression levels and localization of EGFP and 20α-HSD were analyzed in immature testis and in placenta. In support of our recent publication, "Characterization of transgenic mice expressing EGFP under control of monkey 20α-hydroxysteroid dehydrogenase promoter" (Park et al., 2018) [1], it was important to characterize the function of EGFP and 20α-HSD in the ovarian luteal cells of tg mice. Here, the expression of EGFP and 20α-HSD in immature testis and placenta are presented. The expression level of EGFP and 20α-HSD were detected in the testes 1 week after birth, and increased dramatically at 8 weeks. Both of proteins strongly detected in the placenta on days 14, 16, and 18 of pregnancy. Immunohistochemical analysis revealed that EGFP was detected in the seminiferous epithelium and 20α-HSD was specifically localized in the seminiferous tubule at 8 weeks.
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spelling pubmed-59978992018-06-13 Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice Yoo, Mi-Hyang Park, Chae-Won Byambaragchaa, Munkhzaya Kang, Myung-Hwa Min, Kwan-Sik Data Brief Cell biology In order to investigate the function of monkey 20α-hydroxysteroid dehydrogenase (20α-HSD), transgenic mice (tg) were produced, expressing enhanced green fluorescent protein (EGFP) under the control of the monkey 20α-HSD promoter. The expression levels and localization of EGFP and 20α-HSD were analyzed in immature testis and in placenta. In support of our recent publication, "Characterization of transgenic mice expressing EGFP under control of monkey 20α-hydroxysteroid dehydrogenase promoter" (Park et al., 2018) [1], it was important to characterize the function of EGFP and 20α-HSD in the ovarian luteal cells of tg mice. Here, the expression of EGFP and 20α-HSD in immature testis and placenta are presented. The expression level of EGFP and 20α-HSD were detected in the testes 1 week after birth, and increased dramatically at 8 weeks. Both of proteins strongly detected in the placenta on days 14, 16, and 18 of pregnancy. Immunohistochemical analysis revealed that EGFP was detected in the seminiferous epithelium and 20α-HSD was specifically localized in the seminiferous tubule at 8 weeks. Elsevier 2018-05-19 /pmc/articles/PMC5997899/ /pubmed/29900362 http://dx.doi.org/10.1016/j.dib.2018.05.069 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Cell biology
Yoo, Mi-Hyang
Park, Chae-Won
Byambaragchaa, Munkhzaya
Kang, Myung-Hwa
Min, Kwan-Sik
Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title_full Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title_fullStr Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title_full_unstemmed Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title_short Data on the localization of EGFP and 20α-hydroxysteroid dehydrogenase (20α-HSD) in the placenta and testes of transgenic mice
title_sort data on the localization of egfp and 20α-hydroxysteroid dehydrogenase (20α-hsd) in the placenta and testes of transgenic mice
topic Cell biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997899/
https://www.ncbi.nlm.nih.gov/pubmed/29900362
http://dx.doi.org/10.1016/j.dib.2018.05.069
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