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Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey

Honeydew honey is increasingly valued due to its pronounced antibacterial potential; however, the underlying mechanism and compounds responsible for the strong antibacterial activity of honeydew honey are still unknown. The aim of this study was to investigate the inhibition of bacterial growth of 2...

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Autores principales: Bucekova, Marcela, Buriova, Monika, Pekarik, Ladislav, Majtan, Viktor, Majtan, Juraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998132/
https://www.ncbi.nlm.nih.gov/pubmed/29899462
http://dx.doi.org/10.1038/s41598-018-27449-3
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author Bucekova, Marcela
Buriova, Monika
Pekarik, Ladislav
Majtan, Viktor
Majtan, Juraj
author_facet Bucekova, Marcela
Buriova, Monika
Pekarik, Ladislav
Majtan, Viktor
Majtan, Juraj
author_sort Bucekova, Marcela
collection PubMed
description Honeydew honey is increasingly valued due to its pronounced antibacterial potential; however, the underlying mechanism and compounds responsible for the strong antibacterial activity of honeydew honey are still unknown. The aim of this study was to investigate the inhibition of bacterial growth of 23 honeydew honey samples. Activity of bee-derived glucose oxidase (GOX) enzyme, the content of defensin-1 (Def-1) and hydrogen peroxide (H(2)O(2)), and total polyphenol content were determined in the 23 honey samples. Our results demonstrated that antibacterial activity of honeydew honey was equivalent to medical-grade manuka and kanuka honey and was abolished by catalase. Although H(2)O(2) is an important factor in the inhibition of bacterial growth, polyphenolic compounds and their interaction with H(2)O(2) are the key factors responsible for high antibacterial activity of honeydew honey. In addition, our results indicated that the antibacterial activity of honeydew honey is not dependent on GOX-mediated production of H(2)O(2) or the presence of Def-1.
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spelling pubmed-59981322018-06-21 Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey Bucekova, Marcela Buriova, Monika Pekarik, Ladislav Majtan, Viktor Majtan, Juraj Sci Rep Article Honeydew honey is increasingly valued due to its pronounced antibacterial potential; however, the underlying mechanism and compounds responsible for the strong antibacterial activity of honeydew honey are still unknown. The aim of this study was to investigate the inhibition of bacterial growth of 23 honeydew honey samples. Activity of bee-derived glucose oxidase (GOX) enzyme, the content of defensin-1 (Def-1) and hydrogen peroxide (H(2)O(2)), and total polyphenol content were determined in the 23 honey samples. Our results demonstrated that antibacterial activity of honeydew honey was equivalent to medical-grade manuka and kanuka honey and was abolished by catalase. Although H(2)O(2) is an important factor in the inhibition of bacterial growth, polyphenolic compounds and their interaction with H(2)O(2) are the key factors responsible for high antibacterial activity of honeydew honey. In addition, our results indicated that the antibacterial activity of honeydew honey is not dependent on GOX-mediated production of H(2)O(2) or the presence of Def-1. Nature Publishing Group UK 2018-06-13 /pmc/articles/PMC5998132/ /pubmed/29899462 http://dx.doi.org/10.1038/s41598-018-27449-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bucekova, Marcela
Buriova, Monika
Pekarik, Ladislav
Majtan, Viktor
Majtan, Juraj
Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title_full Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title_fullStr Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title_full_unstemmed Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title_short Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
title_sort phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998132/
https://www.ncbi.nlm.nih.gov/pubmed/29899462
http://dx.doi.org/10.1038/s41598-018-27449-3
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