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Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica
Oxidative stress is one of the strongest toxic factors in nature: it can harm or even kill cells. Cellular means of subverting the toxicity of oxidative stress are important for the success of infectious diseases. Many types of bacterium inhabit the intestine, where they can encounter pathogens. Dur...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998147/ https://www.ncbi.nlm.nih.gov/pubmed/29899530 http://dx.doi.org/10.1038/s41598-018-27086-w |
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author | Varet, Hugo Shaulov, Yana Sismeiro, Odile Trebicz-Geffen, Meirav Legendre, Rachel Coppée, Jean-Yves Ankri, Serge Guillen, Nancy |
author_facet | Varet, Hugo Shaulov, Yana Sismeiro, Odile Trebicz-Geffen, Meirav Legendre, Rachel Coppée, Jean-Yves Ankri, Serge Guillen, Nancy |
author_sort | Varet, Hugo |
collection | PubMed |
description | Oxidative stress is one of the strongest toxic factors in nature: it can harm or even kill cells. Cellular means of subverting the toxicity of oxidative stress are important for the success of infectious diseases. Many types of bacterium inhabit the intestine, where they can encounter pathogens. During oxidative stress, we analyzed the interplay between an intestinal parasite (the pathogenic amoeba Entamoeba histolytica - the agent of amoebiasis) and enteric bacteria (microbiome residents, pathogens and probiotics). We found that live enteric bacteria protected E. histolytica against oxidative stress. By high-throughput RNA sequencing, two amoebic regulatory modes were observed with enteric bacteria but not with probiotics. The first controls essential elements of homeostasis, and the second the levels of factors required for amoeba survival. Characteristic genes of both modes have been acquired by the amoebic genome through lateral transfer from the bacterial kingdom (e.g. glycolytic enzymes and leucine-rich proteins). Members of the leucine-rich are homologous to proteins from anti-bacterial innate immune such as Toll-like receptors. The factors identified here suggest that despite its old age in evolutionary terms, the protozoan E. histolytica displays key characteristics of higher eukaryotes’ innate immune systems indicating that components of innate immunity existed in the common ancestor of plants and animals. |
format | Online Article Text |
id | pubmed-5998147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59981472018-07-13 Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica Varet, Hugo Shaulov, Yana Sismeiro, Odile Trebicz-Geffen, Meirav Legendre, Rachel Coppée, Jean-Yves Ankri, Serge Guillen, Nancy Sci Rep Article Oxidative stress is one of the strongest toxic factors in nature: it can harm or even kill cells. Cellular means of subverting the toxicity of oxidative stress are important for the success of infectious diseases. Many types of bacterium inhabit the intestine, where they can encounter pathogens. During oxidative stress, we analyzed the interplay between an intestinal parasite (the pathogenic amoeba Entamoeba histolytica - the agent of amoebiasis) and enteric bacteria (microbiome residents, pathogens and probiotics). We found that live enteric bacteria protected E. histolytica against oxidative stress. By high-throughput RNA sequencing, two amoebic regulatory modes were observed with enteric bacteria but not with probiotics. The first controls essential elements of homeostasis, and the second the levels of factors required for amoeba survival. Characteristic genes of both modes have been acquired by the amoebic genome through lateral transfer from the bacterial kingdom (e.g. glycolytic enzymes and leucine-rich proteins). Members of the leucine-rich are homologous to proteins from anti-bacterial innate immune such as Toll-like receptors. The factors identified here suggest that despite its old age in evolutionary terms, the protozoan E. histolytica displays key characteristics of higher eukaryotes’ innate immune systems indicating that components of innate immunity existed in the common ancestor of plants and animals. Nature Publishing Group UK 2018-06-13 /pmc/articles/PMC5998147/ /pubmed/29899530 http://dx.doi.org/10.1038/s41598-018-27086-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Varet, Hugo Shaulov, Yana Sismeiro, Odile Trebicz-Geffen, Meirav Legendre, Rachel Coppée, Jean-Yves Ankri, Serge Guillen, Nancy Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title | Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title_full | Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title_fullStr | Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title_full_unstemmed | Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title_short | Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica |
title_sort | enteric bacteria boost defences against oxidative stress in entamoeba histolytica |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998147/ https://www.ncbi.nlm.nih.gov/pubmed/29899530 http://dx.doi.org/10.1038/s41598-018-27086-w |
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