Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies

Gene editing protocols often require the use of a subcloning step to isolate successfully edited cells, the behavior of which is then compared to the aggregate parental population and/or other non-edited subclones. Here we demonstrate that the inherent functional heterogeneity present in many cell l...

Descripción completa

Detalles Bibliográficos
Autores principales: Olive, Jessica F., Qin, Yuanbo, DeCristo, Molly J., Laszewski, Tyler, Greathouse, Frances, McAllister, Sandra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999218/
https://www.ncbi.nlm.nih.gov/pubmed/29897959
http://dx.doi.org/10.1371/journal.pone.0198790

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999218/
https://www.ncbi.nlm.nih.gov/pubmed/29897959
http://dx.doi.org/10.1371/journal.pone.0198790

Ejemplares similares