Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

Plant protoplasts are useful for assessing the efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) mutagenesis. We improved the process of protoplast isolation and transfection of several plant species. We also developed a method to iso...

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Detalles Bibliográficos
Autores principales: Lin, Choun‐Sea, Hsu, Chen‐Tran, Yang, Ling‐Hung, Lee, Lan‐Ying, Fu, Jin‐Yuan, Cheng, Qiao‐Wei, Wu, Fu‐Hui, Hsiao, Han C.‐W., Zhang, Yesheng, Zhang, Ru, Chang, Wan‐Jung, Yu, Chen‐Ting, Wang, Wen, Liao, Li‐Jen, Gelvin, Stanton B., Shih, Ming‐Che
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999315/
https://www.ncbi.nlm.nih.gov/pubmed/29230929
http://dx.doi.org/10.1111/pbi.12870

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