磁流体热疗对体外人肺癌细胞A549的影响

BACKGROUND AND OBJECTIVE: Magnetic fluid hyperthermia (MFH) is a method of heat therapy using nanometer techniques and hyperthermia. It has the advantage of high specificity of targeting. The aim of this study is to detect the effects of MFH induced by an alternating magnetic field on human being carcinoma A549 cells in vitro. METHODS: A human adenocarcinoma cell line A549 was cultured with various concentrations of ferroferric oxide (Fe(3)O(4)) magnetic fluid (1.5-6.0) mg/mL and exposed to an alternative magnetic field (AMF) for 30 min. And then the optical density (OD) of viable cell, cytotocixity index, growth curve of cells, morphologic changes of cell, cell cycle and aposptosis were measured. RESULTS: The proliferation of the A549 cells were remarkably inhibited, the OD value of viable cells decreased and cytotoxity index (CI) increased; Apoptosis of the A549 cells were observed to have cell shrinkage, chromatin condensation, margination, unclear fragmentation and intact cell membrane by light and electron microscopy; The cells were inhibited in the stage S. CONCLUSION: MFH induced by AMF could inhibit the proliferation, which promotes apoptosis and arrest at S stage of the A549 cells.