Cargando…

宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险

BACKGROUND AND OBJECTIVE: It has been proven that the lung cancer mortality rate in Xuanwei County, China was among the highest in the country and has been associated with exposure to indoor smoky coal emissions that contain high levels of polycyclic aromatic hydrocarbons. This risk may be modified...

Descripción completa

Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 中国肺癌杂志编辑部 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999738/
https://www.ncbi.nlm.nih.gov/pubmed/25603868
http://dx.doi.org/10.3779/j.issn.1009-3419.2015.01.03
_version_ 1783331495020068864
collection PubMed
description BACKGROUND AND OBJECTIVE: It has been proven that the lung cancer mortality rate in Xuanwei County, China was among the highest in the country and has been associated with exposure to indoor smoky coal emissions that contain high levels of polycyclic aromatic hydrocarbons. This risk may be modified by variation in genetic polymorphisms and coal subtypes. Our objective was to use molecular epidemiological techniques to investigate the relationship among genetic polymorphisms, coal subtype and lung cancer risk in Xuanwei County. METHODS: On the basis of two population-based case-control studies in residents of Xuanwei County, China, questionnaires covering demographic information, smoking history, family and personal medical history, and information on other variables were administered and buccal cells and sputum samples were collected separately from each subject enrolled to extract DNA. GST superfamily, AKR1C3 superfamily, OGG1 superfamily and other genotype were scanned by useing PCR method. ORs and 95%CIs were used to estimate the association between genotypes, coal subtypes and lung cancer risk factors by conditional Logistic regression using Statistical Analysis Software. RESULTS: Compared with subjects who using smokeless coal or wood, smoky coal use was statistically significantly associated with lung cancer risk (OR=7.7, 95%CI: 4.5-13.3). There was marked heterogeneity in risk estimates for specific subtypes of smoky coal. Estimates were highest for coal from the Laibin (OR=24.8), Longtan (OR=11.6) and Baoshan (OR=6.0) coal types, and lower for coal from other types; the risk within the same subtype of coal in male and female were similar. The GSTM1-null genotype, the AKR1C3 (Ex1-70C > G), OGG1 (Ex6-315C > G) genotypes were closely associated with increased risk of lung cancer in Xuanwei County, and their odds ratios (95%CI) were 2.3 (1.3-4.2), 1.8 (1.0-3.5) and 1.9 (1.1-3.3), respectively. Compared to subjects who with GSTM1-positive and used less than 130 tons of smoky coal during their lifetime, higher risks were closely associated with GSTM1-null and heavier users (≥130 tons), with the OR was 4.9 (95%CI: 1.3-18.2) and 2.7 (95%CI: 1.0-7.4) for female and male, respectively. However, higher risks were only found within female for AKR1C3 (Ex1-70C > G) and OGG1 (Ex6-315C > G), with OR (95%CI)=12.9 (2.2-107.8) and 5.7 (1.1-34.2), respectively. CONCLUSION: Lung cancer risks varied among coal subtypes; however, risks were similar between men and women exposed to the same type of coal. The GSTM1-null genotype may enhance susceptibility to air pollution from indoor smoky coal combustion emissions. AKR1C3 and OGG1 genotypes were significantly associated with higher risk of lung cancer, especially among heavily exposed women.
format Online
Article
Text
id pubmed-5999738
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher 中国肺癌杂志编辑部
record_format MEDLINE/PubMed
spelling pubmed-59997382018-07-06 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险 Zhongguo Fei Ai Za Zhi 临床研究 BACKGROUND AND OBJECTIVE: It has been proven that the lung cancer mortality rate in Xuanwei County, China was among the highest in the country and has been associated with exposure to indoor smoky coal emissions that contain high levels of polycyclic aromatic hydrocarbons. This risk may be modified by variation in genetic polymorphisms and coal subtypes. Our objective was to use molecular epidemiological techniques to investigate the relationship among genetic polymorphisms, coal subtype and lung cancer risk in Xuanwei County. METHODS: On the basis of two population-based case-control studies in residents of Xuanwei County, China, questionnaires covering demographic information, smoking history, family and personal medical history, and information on other variables were administered and buccal cells and sputum samples were collected separately from each subject enrolled to extract DNA. GST superfamily, AKR1C3 superfamily, OGG1 superfamily and other genotype were scanned by useing PCR method. ORs and 95%CIs were used to estimate the association between genotypes, coal subtypes and lung cancer risk factors by conditional Logistic regression using Statistical Analysis Software. RESULTS: Compared with subjects who using smokeless coal or wood, smoky coal use was statistically significantly associated with lung cancer risk (OR=7.7, 95%CI: 4.5-13.3). There was marked heterogeneity in risk estimates for specific subtypes of smoky coal. Estimates were highest for coal from the Laibin (OR=24.8), Longtan (OR=11.6) and Baoshan (OR=6.0) coal types, and lower for coal from other types; the risk within the same subtype of coal in male and female were similar. The GSTM1-null genotype, the AKR1C3 (Ex1-70C > G), OGG1 (Ex6-315C > G) genotypes were closely associated with increased risk of lung cancer in Xuanwei County, and their odds ratios (95%CI) were 2.3 (1.3-4.2), 1.8 (1.0-3.5) and 1.9 (1.1-3.3), respectively. Compared to subjects who with GSTM1-positive and used less than 130 tons of smoky coal during their lifetime, higher risks were closely associated with GSTM1-null and heavier users (≥130 tons), with the OR was 4.9 (95%CI: 1.3-18.2) and 2.7 (95%CI: 1.0-7.4) for female and male, respectively. However, higher risks were only found within female for AKR1C3 (Ex1-70C > G) and OGG1 (Ex6-315C > G), with OR (95%CI)=12.9 (2.2-107.8) and 5.7 (1.1-34.2), respectively. CONCLUSION: Lung cancer risks varied among coal subtypes; however, risks were similar between men and women exposed to the same type of coal. The GSTM1-null genotype may enhance susceptibility to air pollution from indoor smoky coal combustion emissions. AKR1C3 and OGG1 genotypes were significantly associated with higher risk of lung cancer, especially among heavily exposed women. 中国肺癌杂志编辑部 2015-01-20 /pmc/articles/PMC5999738/ /pubmed/25603868 http://dx.doi.org/10.3779/j.issn.1009-3419.2015.01.03 Text en 版权所有©《中国肺癌杂志》编辑部2015 https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) License. See: https://creativecommons.org/licenses/by/3.0/
spellingShingle 临床研究
宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title_full 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title_fullStr 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title_full_unstemmed 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title_short 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
title_sort 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险
topic 临床研究
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999738/
https://www.ncbi.nlm.nih.gov/pubmed/25603868
http://dx.doi.org/10.3779/j.issn.1009-3419.2015.01.03
work_keys_str_mv AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn
AT xuānwēifèiáifēnziliúxíngbìngxuéyánjiūméizhǒngjīyīnxíngyǔfèiáifēngxiǎn