宣威肺癌患者肺组织中无机物的赋存与NF-κB信号通路激活以及iNOS表达的相关研究

BACKGROUND AND OBJECTIVE: Indoor air pollution induces asthma, leads to chronic obstructive pulmonary disease, and may promote lung cancer. Our previous studies found that the accumulation of inorganic particulate mater that is due to indoor air pollution can lead to damage to alveolar cells and activation of signaling pathway, and ultimately provoke tumorigenesis. Te aim of this study is to explore the accumulation of inorganics and activation of nuclear factor κB (NF-κB)-inducible nitric oxide synthase (iNOS) signaling pathway of lung tissue in Xuanwei lung cancer patients. METHODS: From December 2013 to November 2014, 48 cases Xuanwei patients with lung cancer who underwent surgical treatment from the Tird Afliated Hospital of Kunming Medical University were enrolled in this study and compared with lung cancer patients from other regions. Te ultrastructure of postoperative specimens was observed by transmission electron microscopy (TEM) to explore the occurrence of inorganic particles. Serum cytokines were analyzed. Ten, the expression levels of NF-κB-p65 protein and iNOS protein in postoperative specimens was explored by immunohistochemistry and Western blot. Finally, 8-OHdG accumulation in lung cancer tissues and urine was measured. RESULTS: A large number of nanoscale inorganics were observed in alveolar type Ⅱ cells and macrophages located in adjacent tissues of lung cancer with Xuanwei patients. Silicon (Si) content was found in inorganic elemental analysis. Te serum interleukin (IL)-1β levels (31.50±19.16) pg/mL of Xuanwei lung-cancer patients were remarkably higher than those from other regions (11.33±6.94) pg/mL (P < 0.01), with statistically signifcant difference. Te pathological tissues of Xuanwei lung-cancer patients express NF-κB-p65, and iNOS expression were signifcantly higher than those of patients from non-Xuanwei regions. No signifcant di?erence was found between cancerous and normal adjacent tissues. Xuanwei lung-cancer tissues and urine 8-OHdG level (40.124±8.597) ng/mgCr were signifcantly higher than those of patients from other regions (25.673±7.986) ng/mg Cr (P < 0.05), with statistically signifcant di?erence. CONCLUSION: Te accumulation of inorganics and the activation of NF-κB-iNOS signaling pathway may contribute to Xuanwei lung cancer.