筛选高表达CPEΔN的H1299肺癌细胞株

BACKGROUND AND OBJECTIVE: Te N-terminal truncated carboxypeptidase E (CPEΔN) protein is a novel biomarker of tumor metastasis. Tis study screened the H1299 cell line with a highly expressed CPEΔN gene for in vivo imaging experiment. METHODS: Human CPEΔN gene was cloned into the luciferase lentiviral vector. H1299 cells transduced with CPEΔN or control lentiviral vectors were selected with 2 μg/mL puromycin. Te expression of CPEΔN was identifed through Western blot analysis, and luciferase activity was measured using luciferase reporters. RESULTS: Te human CPEΔN lentiviral expression vector was successfully constructed. Te transfection rate of H1299 cells by the lentivirus achieved 80%, with an infection multiplicity of 20. Te H1299 cell line with high CPEΔN (H1299-CPEΔN) expression was established, with an increase in CPEΔN expression by four times compared with the control lentivirus-transfected H1299 cell line (H1299-control). As H1299-CPEΔN and H1299-control can e?ectively decompose luciferase substrates, they can be applied in in vivo imaging. CONCLUSION: H1299-CPEΔN and H1299-control can be used in in vivo imaging experiment for further research on molecular mechanisms and signal transduction to elucidate the role of CPEΔN in lung cancer metastasis.