基因富集及meta分析筛选非小细胞肺癌发生发展关键基因的研究

BACKGROUND AND OBJECTIVE: Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors; however, its causes are still not completely understood. This study was designed to screen the key genes and pathways related to NSCLC ℃currence and development and to establish the scientific foundation for the genetic mechanisms and targeted therapy of NSCLC. METHODS: Both gene set-enrichment analysis (GSEA) and meta-analysis (meta) were used to screen the critical pathways and genes that might be corretacted with the development and progression of lung cancer at the transcription level. RESULTS: Using the GSEA and meta methods, focal adhesion and regulation of actin cytoskeleton were determined to be the more prominent overlapping significant pathways. In the focal adhesion pathway, 31 genes were statistically significant (P < 0.05), whereas in the regulation of actin cytoskeleton pathway, 32 genes were statistically significant (P < 0.05). CONCLUSIONS: The focal adhesion and the regulation of actin cytoskeleton pathways might play important roles in the ℃currence and development of NSCLC. Further studies are needed to determine the biological function for the positiue genes.