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Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring

OBJECTIVE(S): Gestational diabetes increases the risk of congenital heart disease in the offspring, but the molecular mechanism underlying this process remains unclear. Therefore, the current study was conducted to assess the effects of induced gestational diabetes on expression of some involved gen...

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Autores principales: Kermani, Elia Saragard, Nazari, Zahra, Mehdizadeh, Mehdi, Shahbazi, Majid, Golalipour, Mohammad Jafar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000218/
https://www.ncbi.nlm.nih.gov/pubmed/29922434
http://dx.doi.org/10.22038/IJBMS.2018.25116.6233
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author Kermani, Elia Saragard
Nazari, Zahra
Mehdizadeh, Mehdi
Shahbazi, Majid
Golalipour, Mohammad Jafar
author_facet Kermani, Elia Saragard
Nazari, Zahra
Mehdizadeh, Mehdi
Shahbazi, Majid
Golalipour, Mohammad Jafar
author_sort Kermani, Elia Saragard
collection PubMed
description OBJECTIVE(S): Gestational diabetes increases the risk of congenital heart disease in the offspring, but the molecular mechanism underlying this process remains unclear. Therefore, the current study was conducted to assess the effects of induced gestational diabetes on expression of some involved genes in cardiac hypertrophy in the offspring of diabetic rats. MATERIALS AND METHODS: Diabetes was induced in 40 adult Wistar rats by intraperitoneal injection of 45 mg/kg of streptozotocin. The day of appearance of the vaginal plug was assumed as day zero of gestation for inducing diabetes. After pregnancy, the offspring was maintained until they reach the age of 12 weeks. Then, their hearts were excised and were sectioned for molecular study. We analyzed the expression pattern of some hypertrophic genes by the quantitative real-time RT-PCR. RESULTS: The mRNA expression levels of all studied genes including c-jun, c-fos, c-myc, alpha-myosin heavy chain (α-MHC), atrial natriuretic factor (ANF) and β-MHC, which are important in cardiomyocyte hypertrophy, were higher in the offspring of the diabetic group compared to controls. Significant differences were found for β-MHC and c-myc with P<0.01 and for α-MHC and c-fos with P<0.05. CONCLUSION: Gestational diabetes upregulates expression of c-jun, c-fos c-myc, α-MHC, ANF and β-MHC genes that are involved in cardiac hypertrophy in the offspring of diabetic rats.
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spelling pubmed-60002182018-06-19 Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring Kermani, Elia Saragard Nazari, Zahra Mehdizadeh, Mehdi Shahbazi, Majid Golalipour, Mohammad Jafar Iran J Basic Med Sci Original Article OBJECTIVE(S): Gestational diabetes increases the risk of congenital heart disease in the offspring, but the molecular mechanism underlying this process remains unclear. Therefore, the current study was conducted to assess the effects of induced gestational diabetes on expression of some involved genes in cardiac hypertrophy in the offspring of diabetic rats. MATERIALS AND METHODS: Diabetes was induced in 40 adult Wistar rats by intraperitoneal injection of 45 mg/kg of streptozotocin. The day of appearance of the vaginal plug was assumed as day zero of gestation for inducing diabetes. After pregnancy, the offspring was maintained until they reach the age of 12 weeks. Then, their hearts were excised and were sectioned for molecular study. We analyzed the expression pattern of some hypertrophic genes by the quantitative real-time RT-PCR. RESULTS: The mRNA expression levels of all studied genes including c-jun, c-fos, c-myc, alpha-myosin heavy chain (α-MHC), atrial natriuretic factor (ANF) and β-MHC, which are important in cardiomyocyte hypertrophy, were higher in the offspring of the diabetic group compared to controls. Significant differences were found for β-MHC and c-myc with P<0.01 and for α-MHC and c-fos with P<0.05. CONCLUSION: Gestational diabetes upregulates expression of c-jun, c-fos c-myc, α-MHC, ANF and β-MHC genes that are involved in cardiac hypertrophy in the offspring of diabetic rats. Mashhad University of Medical Sciences 2018-05 /pmc/articles/PMC6000218/ /pubmed/29922434 http://dx.doi.org/10.22038/IJBMS.2018.25116.6233 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kermani, Elia Saragard
Nazari, Zahra
Mehdizadeh, Mehdi
Shahbazi, Majid
Golalipour, Mohammad Jafar
Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title_full Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title_fullStr Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title_full_unstemmed Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title_short Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
title_sort gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000218/
https://www.ncbi.nlm.nih.gov/pubmed/29922434
http://dx.doi.org/10.22038/IJBMS.2018.25116.6233
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