人长链非编码RNA基因SPRY4-ITl对肺癌细胞A549侵袭和迁移能力的影响

BACKGROUND AND OBJECTIVE: The abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. The aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migration of A549 cells. METHODS: The levels of SPRY4-IT1 in A549 was detected by real-time PCR. The effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. The expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot. RESULTS: The invasion and migration of A549 cells were increased after SPRY4-IT1 over-expression. The cell spaces were narrower after SPRY4-IT1 over-expression in the wound healing assay. Transwell assays showed that the numbers of transmembrane A549 cells were higher in SPRY4-IT1 over-expression group than control group (P < 0.05). Meanwhile, over-expression of SPRY4-IT1 reduced the expression of matrix metalloproteinase (MMP)-2 and MMP-9. CONCLUSION: Over-expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation.