MicroRNA-155对人肺癌95D细胞生长的影响

BACKGROUND AND OBJECTIVE: Recent studies suggest that miR-155 is involved in lung tumorgenesis, whereas the precise mechanism has not yet been characterized. The aim of this study is to investigate the effects of overexpression of miR-155 on the growth of human lung cancer 95D cells in vitro and its possible mechanism, and thus to provide experimental evidence for further researching on the role of miR-155 in the pathogenesis and development of lung cancer. METHODS: miR-155 mimics control and miR-155 mimics were tranfected into human lung cancer 95D cells by FuGENE(®) HD Transfection Reagent respectively in vitro. The relative expression level of miR-155 in 95D cells was determined using specifc probe of real-time PCR afer transfection. The proliferation of 95D cells was detected by MT assay. The cell cycle was analyzed by flow cytometry. The expression of SOS1 protein was measured by Western blot. RESULTS: Compared with control groups, the expression level of miR-155 was signifcantly increased in miR-155 mimics transfected group (P < 0.05). The proliferation of miR-155-transfected 95D cells was signifcantly inhibited (P < 0.05). The percentage of G(0)/G(1) phase cells was increased signifcantly in miR-155-transfected 95D cells, while the percentage of S phase was remarkably reduced (P < 0.05). Furthermore, the expression of SOS1 in miR-155-transfected 95D cells was signifcantly down-regulated (P < 0.05). CONCLUSION: miR-155 could signifcantly inhibit the growth of human lung cancer 95D cells in vitro, which might be closely related to miR-155 induced G(0)/G(1) phase arrest.