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胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值

BACKGROUND AND OBJECTIVE: Carbonic anhydrase IX (CAIX) is widely expressed in a variety of malignant tumors, including-lung cancer. Our previous study has shown that the serum level of soluble form of carbonic anhydrase IX (s-CAIX) was significantly higher in patients with lung cancer than that in t...

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Formato: Online Artículo Texto
Lenguaje:English
Publicado: 中国肺癌杂志编辑部 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000318/
https://www.ncbi.nlm.nih.gov/pubmed/26582225
http://dx.doi.org/10.3779/j.issn.1009-3419.2015.11.06
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collection PubMed
description BACKGROUND AND OBJECTIVE: Carbonic anhydrase IX (CAIX) is widely expressed in a variety of malignant tumors, including-lung cancer. Our previous study has shown that the serum level of soluble form of carbonic anhydrase IX (s-CAIX) was significantly higher in patients with lung cancer than that in the healthy group. The aim of this study is to detect the s-CAIX level in the pleural effusion and its sediment, and to evaluate the significance of CAIX detection in the diagnosis of lung cancer. METHODS: The s-CAIX level in pleural effusion of 29 lung cancer patients and 27 patients with tuberculosis was detected by ELISA. The expression of CAIX in the pleural effusion sediment of 21 lung cancer patients with malignant pleural effusion and 6 patients with benign pleural effusion was examined by immunohistochemistry. With pathological diagnosis as the gold standard, receiver operating characteristic (ROC) curve of pleural effusion s-CAIX was established for the diagnosis of lung cancer with malignant pleural effusion. RESULTS: The s-CAIX level in the malignant pleural effusion was significantly higher than that in the tuberculosis group (P < 0.05). The AUC of pleural effusion s-CAIX level was 0.761. At a threshold level of 109.135 pg/mL, sensitivity and specificity were 92.3% and 58.3%, respectively. The CAIX expression in all samples of the benign pleural effusion sediment was negative. The positive rate of CAIX expression in malignant pleural effusion sediment was 66.67%. CONCLUSION: Detection of CAIX in the pleural effusion and its sediment exhibits high sensitivity and specificity, and is helpful in diagnosis of lung cancer with malignant pleural effusion.
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spelling pubmed-60003182018-07-06 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值 Zhongguo Fei Ai Za Zhi 临床研究 BACKGROUND AND OBJECTIVE: Carbonic anhydrase IX (CAIX) is widely expressed in a variety of malignant tumors, including-lung cancer. Our previous study has shown that the serum level of soluble form of carbonic anhydrase IX (s-CAIX) was significantly higher in patients with lung cancer than that in the healthy group. The aim of this study is to detect the s-CAIX level in the pleural effusion and its sediment, and to evaluate the significance of CAIX detection in the diagnosis of lung cancer. METHODS: The s-CAIX level in pleural effusion of 29 lung cancer patients and 27 patients with tuberculosis was detected by ELISA. The expression of CAIX in the pleural effusion sediment of 21 lung cancer patients with malignant pleural effusion and 6 patients with benign pleural effusion was examined by immunohistochemistry. With pathological diagnosis as the gold standard, receiver operating characteristic (ROC) curve of pleural effusion s-CAIX was established for the diagnosis of lung cancer with malignant pleural effusion. RESULTS: The s-CAIX level in the malignant pleural effusion was significantly higher than that in the tuberculosis group (P < 0.05). The AUC of pleural effusion s-CAIX level was 0.761. At a threshold level of 109.135 pg/mL, sensitivity and specificity were 92.3% and 58.3%, respectively. The CAIX expression in all samples of the benign pleural effusion sediment was negative. The positive rate of CAIX expression in malignant pleural effusion sediment was 66.67%. CONCLUSION: Detection of CAIX in the pleural effusion and its sediment exhibits high sensitivity and specificity, and is helpful in diagnosis of lung cancer with malignant pleural effusion. 中国肺癌杂志编辑部 2015-11-20 /pmc/articles/PMC6000318/ /pubmed/26582225 http://dx.doi.org/10.3779/j.issn.1009-3419.2015.11.06 Text en 版权所有©《中国肺癌杂志》编辑部2015 https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) License. See: https://creativecommons.org/licenses/by/3.0/
spellingShingle 临床研究
胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title_full 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title_fullStr 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title_full_unstemmed 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title_short 胸腔积液及沉淀物碳酸酐酶IX检测对肺癌的诊断价值
title_sort 胸腔积液及沉淀物碳酸酐酶ix检测对肺癌的诊断价值
topic 临床研究
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000318/
https://www.ncbi.nlm.nih.gov/pubmed/26582225
http://dx.doi.org/10.3779/j.issn.1009-3419.2015.11.06
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