全基因组芯片筛查非小细胞肺癌组织多药耐药相关基因

BACKGROUND AND OBJECTIVE: The aim of this study is to screen for multi-drug resistance-related genes of human non-small cell lung cancer (NSCLC), and provide the evidences for drug-sensitive predicting genes of different NSCLC patients treated with chemotherapeutic drugs. METHODS: Sensitivity and inhibition ratio of five antitumor drugs (NVB, GEM, TAL, DOC, CDDP) on 75 fresh NSCLC samples from different individuals were studied by means of culturing primary tumor cells and MTT assay. After the five chemotherapeutic drugs were used, multi-drug resistance-related genes of NSCLC with cDNA microarry on the samples which were all high sensitive and those resistant were screened. RESULTS: cDNA microarray analysis screened out 212 genes, 168 of which were up-regulated while the other 44 were down-regulated in the group of highly sensitive compared with the group of resistance. CONCLUSION: The multi-drug resistance of NSCLC may be correlative with the 212 genes screened by cDNA microarray; the detailed mechanisms of the genes still need to be detected in the future.