In Silico Analysis of the Quorum Sensing Metagenome in Environmental Biofilm Samples

Quorum sensing (QS) is a sophisticated cell to cell signaling mechanism mediated by small diffusible molecules called “autoinducers.” This phenomenon is well studied in bacteria, where different QS systems are described that differ between Gram-negative and Gram-positive bacteria. However, a common system to these groups was discovered, the autoinducer 2. QS has implications in biofilm formation, where the application of metagenomic techniques to study these phenomena may be useful to understand the communication networks established by the different components of the community, and to discover new targets for microbial control. Here we present an in silico screening of QS proteins in all publicly available biofilm metagenomes from the JGI database. We performed sequence, conserved motifs, phylogenetic, and three-dimensional structure analyses of the candidates, resulting in an effective strategy to search QS proteins in metagenomes sequences. The number of QS proteins present in each sample, and its phylogenetic affiliation, was clearly related to the bacterial diversity and the origin of the biofilm. The samples isolated from natural habitats presented clear differences with those from artificial habitats. Interesting findings have been made in the abundance of LuxR-like proteins finding an unbalanced ratio between the synthases and the receptor proteins in Bacteroidetes bacteria, pointing out the existence of “cheaters” in this group. Moreover, we have shown the presence of the LuxI/R QS system in bacteria from the Nitrospira taxonomic group. Finally, some undescribed proteins from the HdtS family have been found in Gamma-proteobacteria.