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Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy
BACKGROUND: Along with trophoblast elongation (Days 10 to 12), estradiol is secreted in increasing amounts for recognition of pregnancy. Endometrial secretions driven by ovarian progesterone and conceptus signals are essential for conceptus growth and development. Results of transcriptome analyses o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000939/ https://www.ncbi.nlm.nih.gov/pubmed/29898663 http://dx.doi.org/10.1186/s12864-018-4855-y |
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author | Zeng, Shuqin Bick, Jochen Ulbrich, Susanne E. Bauersachs, Stefan |
author_facet | Zeng, Shuqin Bick, Jochen Ulbrich, Susanne E. Bauersachs, Stefan |
author_sort | Zeng, Shuqin |
collection | PubMed |
description | BACKGROUND: Along with trophoblast elongation (Days 10 to 12), estradiol is secreted in increasing amounts for recognition of pregnancy. Endometrial secretions driven by ovarian progesterone and conceptus signals are essential for conceptus growth and development. Results of transcriptome analyses of whole endometrial tissue samples in the pig indicated the need for cell type-specific endometrial gene expression analysis for a better understanding of transcriptome changes associated with establishment of pregnancy. RESULTS: The most distinct transcriptome profile and the majority of differentially expressed genes (DEGs) were identified in luminal epithelium (LE). Many DEGs were found only in the cell type-specific analysis. The functional classification of DEGs identified in specific endometrial cell types revealed various distinct functions and pathways. Genes related to immune activation, estrogen signaling pathway, embryo development, and cell proliferation were upregulated in LE of pregnant gilts. Genes involved in sterol biosynthetic and metabolic processes and extracellular matrix were upregulated in stroma. Genes associated with cell communication such as via exosomes and vesicles were found as differential in LE, glandular epithelium (GE), and stroma (S). CONCLUSIONS: This study revealed that conceptus signals induce different transcriptomic regulations in the endometrial compartments/cell types related to their specific function during recognition and establishment of pregnancy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4855-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6000939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60009392018-06-25 Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy Zeng, Shuqin Bick, Jochen Ulbrich, Susanne E. Bauersachs, Stefan BMC Genomics Research Article BACKGROUND: Along with trophoblast elongation (Days 10 to 12), estradiol is secreted in increasing amounts for recognition of pregnancy. Endometrial secretions driven by ovarian progesterone and conceptus signals are essential for conceptus growth and development. Results of transcriptome analyses of whole endometrial tissue samples in the pig indicated the need for cell type-specific endometrial gene expression analysis for a better understanding of transcriptome changes associated with establishment of pregnancy. RESULTS: The most distinct transcriptome profile and the majority of differentially expressed genes (DEGs) were identified in luminal epithelium (LE). Many DEGs were found only in the cell type-specific analysis. The functional classification of DEGs identified in specific endometrial cell types revealed various distinct functions and pathways. Genes related to immune activation, estrogen signaling pathway, embryo development, and cell proliferation were upregulated in LE of pregnant gilts. Genes involved in sterol biosynthetic and metabolic processes and extracellular matrix were upregulated in stroma. Genes associated with cell communication such as via exosomes and vesicles were found as differential in LE, glandular epithelium (GE), and stroma (S). CONCLUSIONS: This study revealed that conceptus signals induce different transcriptomic regulations in the endometrial compartments/cell types related to their specific function during recognition and establishment of pregnancy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4855-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-06-14 /pmc/articles/PMC6000939/ /pubmed/29898663 http://dx.doi.org/10.1186/s12864-018-4855-y Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zeng, Shuqin Bick, Jochen Ulbrich, Susanne E. Bauersachs, Stefan Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title | Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title_full | Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title_fullStr | Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title_full_unstemmed | Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title_short | Cell type-specific analysis of transcriptome changes in the porcine endometrium on Day 12 of pregnancy |
title_sort | cell type-specific analysis of transcriptome changes in the porcine endometrium on day 12 of pregnancy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000939/ https://www.ncbi.nlm.nih.gov/pubmed/29898663 http://dx.doi.org/10.1186/s12864-018-4855-y |
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