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Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000982/ https://www.ncbi.nlm.nih.gov/pubmed/29997870 http://dx.doi.org/10.1039/c8sc01638k |
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author | Hickey, David P. Lim, Koun Cai, Rong Patterson, Ashlea R. Yuan, Mengwei Sahin, Selmihan Abdellaoui, Sofiene Minteer, Shelley D. |
author_facet | Hickey, David P. Lim, Koun Cai, Rong Patterson, Ashlea R. Yuan, Mengwei Sahin, Selmihan Abdellaoui, Sofiene Minteer, Shelley D. |
author_sort | Hickey, David P. |
collection | PubMed |
description | Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O(2) by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N(2) to NH(3). |
format | Online Article Text |
id | pubmed-6000982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-60009822018-07-11 Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2) Hickey, David P. Lim, Koun Cai, Rong Patterson, Ashlea R. Yuan, Mengwei Sahin, Selmihan Abdellaoui, Sofiene Minteer, Shelley D. Chem Sci Chemistry Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O(2) by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N(2) to NH(3). Royal Society of Chemistry 2018-05-14 /pmc/articles/PMC6000982/ /pubmed/29997870 http://dx.doi.org/10.1039/c8sc01638k Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0) |
spellingShingle | Chemistry Hickey, David P. Lim, Koun Cai, Rong Patterson, Ashlea R. Yuan, Mengwei Sahin, Selmihan Abdellaoui, Sofiene Minteer, Shelley D. Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2) |
title | Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
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title_full | Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
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title_fullStr | Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
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title_full_unstemmed | Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
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title_short | Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
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title_sort | pyrene hydrogel for promoting direct bioelectrochemistry: atp-independent electroenzymatic reduction of n(2) |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000982/ https://www.ncbi.nlm.nih.gov/pubmed/29997870 http://dx.doi.org/10.1039/c8sc01638k |
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