Cargando…

Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)

Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytic...

Descripción completa

Detalles Bibliográficos
Autores principales: Hickey, David P., Lim, Koun, Cai, Rong, Patterson, Ashlea R., Yuan, Mengwei, Sahin, Selmihan, Abdellaoui, Sofiene, Minteer, Shelley D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000982/
https://www.ncbi.nlm.nih.gov/pubmed/29997870
http://dx.doi.org/10.1039/c8sc01638k
_version_ 1783331881552445440
author Hickey, David P.
Lim, Koun
Cai, Rong
Patterson, Ashlea R.
Yuan, Mengwei
Sahin, Selmihan
Abdellaoui, Sofiene
Minteer, Shelley D.
author_facet Hickey, David P.
Lim, Koun
Cai, Rong
Patterson, Ashlea R.
Yuan, Mengwei
Sahin, Selmihan
Abdellaoui, Sofiene
Minteer, Shelley D.
author_sort Hickey, David P.
collection PubMed
description Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O(2) by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N(2) to NH(3).
format Online
Article
Text
id pubmed-6000982
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-60009822018-07-11 Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2) Hickey, David P. Lim, Koun Cai, Rong Patterson, Ashlea R. Yuan, Mengwei Sahin, Selmihan Abdellaoui, Sofiene Minteer, Shelley D. Chem Sci Chemistry Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O(2) by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N(2) to NH(3). Royal Society of Chemistry 2018-05-14 /pmc/articles/PMC6000982/ /pubmed/29997870 http://dx.doi.org/10.1039/c8sc01638k Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Hickey, David P.
Lim, Koun
Cai, Rong
Patterson, Ashlea R.
Yuan, Mengwei
Sahin, Selmihan
Abdellaoui, Sofiene
Minteer, Shelley D.
Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title_full Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title_fullStr Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title_full_unstemmed Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title_short Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N(2)
title_sort pyrene hydrogel for promoting direct bioelectrochemistry: atp-independent electroenzymatic reduction of n(2)
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000982/
https://www.ncbi.nlm.nih.gov/pubmed/29997870
http://dx.doi.org/10.1039/c8sc01638k
work_keys_str_mv AT hickeydavidp pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT limkoun pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT cairong pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT pattersonashlear pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT yuanmengwei pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT sahinselmihan pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT abdellaouisofiene pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2
AT minteershelleyd pyrenehydrogelforpromotingdirectbioelectrochemistryatpindependentelectroenzymaticreductionofn2