Identification of a Novel 5-HT2 Receptor CDNA in the Ovary tissues of Black Tiger Shrimp (Paneaus Monodon)

The purpose of this experimental study is to isolate a 5-HT receptor from Penaeus monodon. The mRNA were isolated from ovary (stage III ) of the wild broad stock then reserve transcribed to cDNA by using Oligo (dT) primer and superscript III enzyme. The template was amplified by PCR technique, used...

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Detalles Bibliográficos
Autor principal: Mai, Nguyen Thi Phuong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Communications and Publications Division (CPD) of the IFCC 2005
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001052/
https://www.ncbi.nlm.nih.gov/pubmed/29922117
Descripción
Sumario:The purpose of this experimental study is to isolate a 5-HT receptor from Penaeus monodon. The mRNA were isolated from ovary (stage III ) of the wild broad stock then reserve transcribed to cDNA by using Oligo (dT) primer and superscript III enzyme. The template was amplified by PCR technique, used Taq DNA polymerase and two degenerate primers 5-HT-TM2 and 5-HT-TM6, corresponding to the conserved amino acid sequences of invertebrate 5-HT receptors. After cloning, checking positive PCR product, and sequencing analysis revealed an opened frame of 404 acid nucleotides, which was high identity of acid amine coding, and coded for 133 acid amines of protein G. Those result and the 5-HT-TM2 primer expressed in that opened frame were evident presentation of 5-HT2 receptor in P. monodon.

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