Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture image...

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Detalles Bibliográficos
Autores principales: Coelho, Polyana Galvão Bernardes, de Souza, Maria Verônica, Conceição, Lissandro Gonçalves, Viloria, Marlene Isabel Vargas, Bedoya, Sirley Adriana Ortiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Dermatologia 2018
Materias:
Dermatopathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001092/
https://www.ncbi.nlm.nih.gov/pubmed/29924246
http://dx.doi.org/10.1590/abd1806-4841.20187544
Descripción
Sumario:The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.

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