Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture image...

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Autores principales: Coelho, Polyana Galvão Bernardes, de Souza, Maria Verônica, Conceição, Lissandro Gonçalves, Viloria, Marlene Isabel Vargas, Bedoya, Sirley Adriana Ortiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Dermatologia 2018
Materias:
Dermatopathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001092/
https://www.ncbi.nlm.nih.gov/pubmed/29924246
http://dx.doi.org/10.1590/abd1806-4841.20187544
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author Coelho, Polyana Galvão Bernardes
de Souza, Maria Verônica
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
author_facet Coelho, Polyana Galvão Bernardes
de Souza, Maria Verônica
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
author_sort Coelho, Polyana Galvão Bernardes
collection PubMed
description The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
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spelling pubmed-60010922018-06-19 Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy Coelho, Polyana Galvão Bernardes de Souza, Maria Verônica Conceição, Lissandro Gonçalves Viloria, Marlene Isabel Vargas Bedoya, Sirley Adriana Ortiz An Bras Dermatol Dermatopathology The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur. Sociedade Brasileira de Dermatologia 2018 /pmc/articles/PMC6001092/ /pubmed/29924246 http://dx.doi.org/10.1590/abd1806-4841.20187544 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivative License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited and the work is not changed in any way.
spellingShingle Dermatopathology
Coelho, Polyana Galvão Bernardes
de Souza, Maria Verônica
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_full Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_fullStr Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_full_unstemmed Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_short Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_sort evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
topic Dermatopathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001092/
https://www.ncbi.nlm.nih.gov/pubmed/29924246
http://dx.doi.org/10.1590/abd1806-4841.20187544
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