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CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis

CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that are sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity that signals together with a more promiscuous co-receptor....

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Autores principales: Anne, Pauline, Amiguet-Vercher, Amelia, Brandt, Benjamin, Kalmbach, Lothar, Geldner, Niko, Hothorn, Michael, Hardtke, Christian S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001375/
https://www.ncbi.nlm.nih.gov/pubmed/29789310
http://dx.doi.org/10.1242/dev.162354
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author Anne, Pauline
Amiguet-Vercher, Amelia
Brandt, Benjamin
Kalmbach, Lothar
Geldner, Niko
Hothorn, Michael
Hardtke, Christian S.
author_facet Anne, Pauline
Amiguet-Vercher, Amelia
Brandt, Benjamin
Kalmbach, Lothar
Geldner, Niko
Hothorn, Michael
Hardtke, Christian S.
author_sort Anne, Pauline
collection PubMed
description CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that are sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity that signals together with a more promiscuous co-receptor. For most CLEs, biologically relevant (co-)receptors are unknown. The dimer of the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) conditions perception of so-called root-active CLE peptides, the exogenous application of which suppresses root growth by preventing protophloem formation in the meristem. clv2 as well as crn null mutants are resistant to root-active CLE peptides, possibly because CLV2-CRN promotes expression of their cognate receptors. Here, we have identified the CLE-RESISTANT RECEPTOR KINASE (CLERK) gene, which is required for full sensing of root-active CLE peptides in early developing protophloem. CLERK protein can be replaced by its close homologs, SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) and NSP-INTERACTING KINASE 1 (NIK1). Yet neither CLERK nor NIK1 ectodomains interact biochemically with described CLE receptor ectodomains. Consistently, CLERK also acts genetically independently of CLV2-CRN. We, thus, have discovered a novel hub for redundant CLE sensing in the root.
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spelling pubmed-60013752018-06-22 CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis Anne, Pauline Amiguet-Vercher, Amelia Brandt, Benjamin Kalmbach, Lothar Geldner, Niko Hothorn, Michael Hardtke, Christian S. Development Research Article CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that are sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity that signals together with a more promiscuous co-receptor. For most CLEs, biologically relevant (co-)receptors are unknown. The dimer of the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) conditions perception of so-called root-active CLE peptides, the exogenous application of which suppresses root growth by preventing protophloem formation in the meristem. clv2 as well as crn null mutants are resistant to root-active CLE peptides, possibly because CLV2-CRN promotes expression of their cognate receptors. Here, we have identified the CLE-RESISTANT RECEPTOR KINASE (CLERK) gene, which is required for full sensing of root-active CLE peptides in early developing protophloem. CLERK protein can be replaced by its close homologs, SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) and NSP-INTERACTING KINASE 1 (NIK1). Yet neither CLERK nor NIK1 ectodomains interact biochemically with described CLE receptor ectodomains. Consistently, CLERK also acts genetically independently of CLV2-CRN. We, thus, have discovered a novel hub for redundant CLE sensing in the root. The Company of Biologists Ltd 2018-05-15 2018-05-22 /pmc/articles/PMC6001375/ /pubmed/29789310 http://dx.doi.org/10.1242/dev.162354 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Anne, Pauline
Amiguet-Vercher, Amelia
Brandt, Benjamin
Kalmbach, Lothar
Geldner, Niko
Hothorn, Michael
Hardtke, Christian S.
CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title_full CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title_fullStr CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title_full_unstemmed CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title_short CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
title_sort clerk is a novel receptor kinase required for sensing of root-active cle peptides in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001375/
https://www.ncbi.nlm.nih.gov/pubmed/29789310
http://dx.doi.org/10.1242/dev.162354
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