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Transfection of Capsaspora owczarzaki, a close unicellular relative of animals

How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpec...

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Autores principales: Parra-Acero, Helena, Ros-Rocher, Núria, Perez-Posada, Alberto, Kożyczkowska, Aleksandra, Sánchez-Pons, Núria, Nakata, Azusa, Suga, Hiroshi, Najle, Sebastián R., Ruiz-Trillo, Iñaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001378/
https://www.ncbi.nlm.nih.gov/pubmed/29752387
http://dx.doi.org/10.1242/dev.162107
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author Parra-Acero, Helena
Ros-Rocher, Núria
Perez-Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez-Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián R.
Ruiz-Trillo, Iñaki
author_facet Parra-Acero, Helena
Ros-Rocher, Núria
Perez-Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez-Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián R.
Ruiz-Trillo, Iñaki
author_sort Parra-Acero, Helena
collection PubMed
description How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.
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spelling pubmed-60013782018-06-22 Transfection of Capsaspora owczarzaki, a close unicellular relative of animals Parra-Acero, Helena Ros-Rocher, Núria Perez-Posada, Alberto Kożyczkowska, Aleksandra Sánchez-Pons, Núria Nakata, Azusa Suga, Hiroshi Najle, Sebastián R. Ruiz-Trillo, Iñaki Development Techniques and Resources How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity. The Company of Biologists Ltd 2018-05-15 2018-05-23 /pmc/articles/PMC6001378/ /pubmed/29752387 http://dx.doi.org/10.1242/dev.162107 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Techniques and Resources
Parra-Acero, Helena
Ros-Rocher, Núria
Perez-Posada, Alberto
Kożyczkowska, Aleksandra
Sánchez-Pons, Núria
Nakata, Azusa
Suga, Hiroshi
Najle, Sebastián R.
Ruiz-Trillo, Iñaki
Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_full Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_fullStr Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_full_unstemmed Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_short Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
title_sort transfection of capsaspora owczarzaki, a close unicellular relative of animals
topic Techniques and Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001378/
https://www.ncbi.nlm.nih.gov/pubmed/29752387
http://dx.doi.org/10.1242/dev.162107
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