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The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy

The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood. In this work, a series of NaYF(4):Yb(3+), Er(3+) upconversion nanoparticles (UCNP...

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Autores principales: Zhang, Di, Wei, Lin, Zhong, Meile, Xiao, Lehui, Li, Hung-Wing, Wang, Jianfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001388/
https://www.ncbi.nlm.nih.gov/pubmed/29997881
http://dx.doi.org/10.1039/c8sc01828f
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author Zhang, Di
Wei, Lin
Zhong, Meile
Xiao, Lehui
Li, Hung-Wing
Wang, Jianfang
author_facet Zhang, Di
Wei, Lin
Zhong, Meile
Xiao, Lehui
Li, Hung-Wing
Wang, Jianfang
author_sort Zhang, Di
collection PubMed
description The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood. In this work, a series of NaYF(4):Yb(3+), Er(3+) upconversion nanoparticles (UCNPs) with different morphologies and surface coatings were prepared to explore the influence of surface charge and morphology on the cellular internalization process with single-particle fluorescence microscopy. It is found that the higher the surface charge and larger the surface-to-volume ratio of the nanoparticles, the more efficient the cellular uptake will be. Particularly, the surface charge is demonstrated to be the primary influence factor for small sized nanoparticles on the cellular uptake process. By blocking the endocytosis routes with temperature modulation (from 37 to 4 °C) or introduction of chemical inhibitors (dynasore and genistein), multiplexed mechanisms are found to be involved in the cellular uptake process, including clathrin- and caveolae-mediated endocytosis, physical adhesion and penetration, and so on. Moreover, in the aspect of size effect, an energy-dependent endocytosis process plays a more important role for larger size particles. In short, this study presents a pattern of cellular internalization pathway for the nanoparticles with different morphologies and surface charges, which would provide useful information for the development of robust drug delivery systems.
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spelling pubmed-60013882018-07-11 The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy Zhang, Di Wei, Lin Zhong, Meile Xiao, Lehui Li, Hung-Wing Wang, Jianfang Chem Sci Chemistry The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood. In this work, a series of NaYF(4):Yb(3+), Er(3+) upconversion nanoparticles (UCNPs) with different morphologies and surface coatings were prepared to explore the influence of surface charge and morphology on the cellular internalization process with single-particle fluorescence microscopy. It is found that the higher the surface charge and larger the surface-to-volume ratio of the nanoparticles, the more efficient the cellular uptake will be. Particularly, the surface charge is demonstrated to be the primary influence factor for small sized nanoparticles on the cellular uptake process. By blocking the endocytosis routes with temperature modulation (from 37 to 4 °C) or introduction of chemical inhibitors (dynasore and genistein), multiplexed mechanisms are found to be involved in the cellular uptake process, including clathrin- and caveolae-mediated endocytosis, physical adhesion and penetration, and so on. Moreover, in the aspect of size effect, an energy-dependent endocytosis process plays a more important role for larger size particles. In short, this study presents a pattern of cellular internalization pathway for the nanoparticles with different morphologies and surface charges, which would provide useful information for the development of robust drug delivery systems. Royal Society of Chemistry 2018-05-18 /pmc/articles/PMC6001388/ /pubmed/29997881 http://dx.doi.org/10.1039/c8sc01828f Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Zhang, Di
Wei, Lin
Zhong, Meile
Xiao, Lehui
Li, Hung-Wing
Wang, Jianfang
The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title_full The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title_fullStr The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title_full_unstemmed The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title_short The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
title_sort morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001388/
https://www.ncbi.nlm.nih.gov/pubmed/29997881
http://dx.doi.org/10.1039/c8sc01828f
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