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Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery

BACKGROUND: Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigat...

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Autores principales: da Costa, Jimena Ferreira, Ferrarini, Mariana Galvão, Nardelli, Sheila Cristina, Goldenberg, Samuel, Ávila, Andréa Rodrigues, Holetz, Fabíola Barbieri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001583/
https://www.ncbi.nlm.nih.gov/pubmed/29924141
http://dx.doi.org/10.1590/0074-02760170531
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author da Costa, Jimena Ferreira
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
author_facet da Costa, Jimena Ferreira
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
author_sort da Costa, Jimena Ferreira
collection PubMed
description BACKGROUND: Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES: This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS: We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS: TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS: TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.
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spelling pubmed-60015832018-06-19 Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery da Costa, Jimena Ferreira Ferrarini, Mariana Galvão Nardelli, Sheila Cristina Goldenberg, Samuel Ávila, Andréa Rodrigues Holetz, Fabíola Barbieri Mem Inst Oswaldo Cruz Original Article BACKGROUND: Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES: This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS: We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS: TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS: TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism. Instituto Oswaldo Cruz, Ministério da Saúde 2018-06-18 /pmc/articles/PMC6001583/ /pubmed/29924141 http://dx.doi.org/10.1590/0074-02760170531 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
da Costa, Jimena Ferreira
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_full Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_fullStr Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_full_unstemmed Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_short Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_sort trypanosoma cruzi xrna granules colocalise with distinct mrnp granules at the nuclear periphery
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001583/
https://www.ncbi.nlm.nih.gov/pubmed/29924141
http://dx.doi.org/10.1590/0074-02760170531
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