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Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species
Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency. This pathogen has a tractable genetic system; however, gene disruption via electroporation remains difficult, while biolistic transformation is oft...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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American Society for Microbiology
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001613/ https://www.ncbi.nlm.nih.gov/pubmed/29898980 http://dx.doi.org/10.1128/mSphereDirect.00208-18 |