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MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells
Gastric cancer (GC) is one of the most common malignancies with high mortality rate. MiR-152 may exert the function of tumor suppressor by regulating its target gene, including PIK3CA. Nevertheless, all of the described functions are referred explicitly to miR-152-3p, while miR-152-5p as a passenger...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001653/ https://www.ncbi.nlm.nih.gov/pubmed/29904279 http://dx.doi.org/10.7150/ijbs.25272 |
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author | You, Wendao Zhang, Xing Ji, Mengyue Yu, Yang Chen, Chen Xiong, Yujia Liu, Yiting Sun, Yibin Tan, Chenhuan Zhang, Hanshuo Li, Jie Chen, Weichang Li, Rui |
author_facet | You, Wendao Zhang, Xing Ji, Mengyue Yu, Yang Chen, Chen Xiong, Yujia Liu, Yiting Sun, Yibin Tan, Chenhuan Zhang, Hanshuo Li, Jie Chen, Weichang Li, Rui |
author_sort | You, Wendao |
collection | PubMed |
description | Gastric cancer (GC) is one of the most common malignancies with high mortality rate. MiR-152 may exert the function of tumor suppressor by regulating its target gene, including PIK3CA. Nevertheless, all of the described functions are referred explicitly to miR-152-3p, while miR-152-5p as a passenger strand is poorly realized and entirely ignored. We previously selected miR-152-5p as a candidate using cell migration inhibition screening for GC cells and predicted that miR-152-5p might also target PIK3CA. In this study, we found an abnormal proportion of miR-152-3p / miR-152-5p in GC (gastric cancer) tissues and cells and demonstrated that miR-152-5p had poorer stability in GC cells, revealing the possibility that miR-152-5p is abnormally "suppressed" in gastric cancer. We also investigated and confirmed the role of miR-152-5p in GC by a series of experiments, and found that miR-152-5p modulated cell viability, migration, invasion, and cell-cycle progression of human GC cells, and also inhibited tumor growth and metastasis in vivo partially by targeting PIK3CA. More interestingly, it was proved that miR-152-3p and miR-152-5p had synergistic effects on the inhibition of PIK3CA in GC cells. The results of this study suggest that miR-152-5p may act as a tumor suppressor in SGC-7901 gastric cancer cells via targeting PIK3CA. Further, the study provides a novel insight into the roles of miRNA* during carcinogenesis. |
format | Online Article Text |
id | pubmed-6001653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-60016532018-06-14 MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells You, Wendao Zhang, Xing Ji, Mengyue Yu, Yang Chen, Chen Xiong, Yujia Liu, Yiting Sun, Yibin Tan, Chenhuan Zhang, Hanshuo Li, Jie Chen, Weichang Li, Rui Int J Biol Sci Research Paper Gastric cancer (GC) is one of the most common malignancies with high mortality rate. MiR-152 may exert the function of tumor suppressor by regulating its target gene, including PIK3CA. Nevertheless, all of the described functions are referred explicitly to miR-152-3p, while miR-152-5p as a passenger strand is poorly realized and entirely ignored. We previously selected miR-152-5p as a candidate using cell migration inhibition screening for GC cells and predicted that miR-152-5p might also target PIK3CA. In this study, we found an abnormal proportion of miR-152-3p / miR-152-5p in GC (gastric cancer) tissues and cells and demonstrated that miR-152-5p had poorer stability in GC cells, revealing the possibility that miR-152-5p is abnormally "suppressed" in gastric cancer. We also investigated and confirmed the role of miR-152-5p in GC by a series of experiments, and found that miR-152-5p modulated cell viability, migration, invasion, and cell-cycle progression of human GC cells, and also inhibited tumor growth and metastasis in vivo partially by targeting PIK3CA. More interestingly, it was proved that miR-152-3p and miR-152-5p had synergistic effects on the inhibition of PIK3CA in GC cells. The results of this study suggest that miR-152-5p may act as a tumor suppressor in SGC-7901 gastric cancer cells via targeting PIK3CA. Further, the study provides a novel insight into the roles of miRNA* during carcinogenesis. Ivyspring International Publisher 2018-04-30 /pmc/articles/PMC6001653/ /pubmed/29904279 http://dx.doi.org/10.7150/ijbs.25272 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper You, Wendao Zhang, Xing Ji, Mengyue Yu, Yang Chen, Chen Xiong, Yujia Liu, Yiting Sun, Yibin Tan, Chenhuan Zhang, Hanshuo Li, Jie Chen, Weichang Li, Rui MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title | MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title_full | MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title_fullStr | MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title_full_unstemmed | MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title_short | MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cells |
title_sort | mir-152-5p as a microrna passenger strand special functions in human gastric cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001653/ https://www.ncbi.nlm.nih.gov/pubmed/29904279 http://dx.doi.org/10.7150/ijbs.25272 |
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