A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells

The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in mu...

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Autores principales: Chen, Zhizhen, Liu, Jie, Chu, Dafeng, Shan, Yaming, Ma, Guixing, Zhang, Hongmin, Zhang, Xiaohua Douglas, Wang, Pu, Chen, Qiang, Deng, Chuxia, Chen, Weizao, Dimitrov, Dimiter S., Zhao, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2018
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001679/
https://www.ncbi.nlm.nih.gov/pubmed/29910690
http://dx.doi.org/10.7150/ijbs.25928
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author Chen, Zhizhen
Liu, Jie
Chu, Dafeng
Shan, Yaming
Ma, Guixing
Zhang, Hongmin
Zhang, Xiaohua Douglas
Wang, Pu
Chen, Qiang
Deng, Chuxia
Chen, Weizao
Dimitrov, Dimiter S.
Zhao, Qi
author_facet Chen, Zhizhen
Liu, Jie
Chu, Dafeng
Shan, Yaming
Ma, Guixing
Zhang, Hongmin
Zhang, Xiaohua Douglas
Wang, Pu
Chen, Qiang
Deng, Chuxia
Chen, Weizao
Dimitrov, Dimiter S.
Zhao, Qi
author_sort Chen, Zhizhen
collection PubMed
description The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in multiple types of tumors. Here, we have firstly identified a human engineered antibody domain (eAd) from a phage-displayed VH library. The eAd suppressed the signal transduction of IGF-1R mediated by exogenous IGF-I or IGF-II in breast cancer cell lines through neutralizing both IGF-I and IGF-II. It also significantly inhibited the growth of breast cancer cells. Therefore, the anti-IGF-I/II eAd offers an alternative approach to target both the IGF-1R signaling pathways through the inhibition of IGF-I/II.
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spelling pubmed-60016792018-06-15 A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells Chen, Zhizhen Liu, Jie Chu, Dafeng Shan, Yaming Ma, Guixing Zhang, Hongmin Zhang, Xiaohua Douglas Wang, Pu Chen, Qiang Deng, Chuxia Chen, Weizao Dimitrov, Dimiter S. Zhao, Qi Int J Biol Sci Research Paper The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in multiple types of tumors. Here, we have firstly identified a human engineered antibody domain (eAd) from a phage-displayed VH library. The eAd suppressed the signal transduction of IGF-1R mediated by exogenous IGF-I or IGF-II in breast cancer cell lines through neutralizing both IGF-I and IGF-II. It also significantly inhibited the growth of breast cancer cells. Therefore, the anti-IGF-I/II eAd offers an alternative approach to target both the IGF-1R signaling pathways through the inhibition of IGF-I/II. Ivyspring International Publisher 2018-05-21 /pmc/articles/PMC6001679/ /pubmed/29910690 http://dx.doi.org/10.7150/ijbs.25928 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chen, Zhizhen
Liu, Jie
Chu, Dafeng
Shan, Yaming
Ma, Guixing
Zhang, Hongmin
Zhang, Xiaohua Douglas
Wang, Pu
Chen, Qiang
Deng, Chuxia
Chen, Weizao
Dimitrov, Dimiter S.
Zhao, Qi
A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title_full A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title_fullStr A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title_full_unstemmed A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title_short A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
title_sort dual-specific igf-i/ii human engineered antibody domain inhibits igf signaling in breast cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001679/
https://www.ncbi.nlm.nih.gov/pubmed/29910690
http://dx.doi.org/10.7150/ijbs.25928
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