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Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

DNA methylation is one of the most important epigenetic modifications in the regulation of gene transcription. The current gold standard to study this modification is bisulfite sequencing. Although multiple commercial bisulfite treatment kits provide good conversion efficiencies, DNA loss and especi...

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Detalles Bibliográficos
Autores principales:	Kint, Sam, De Spiegelaere, Ward, De Kesel, Jonas, Vandekerckhove, Linos, Van Criekinge, Wim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002050/ https://www.ncbi.nlm.nih.gov/pubmed/29902267 http://dx.doi.org/10.1371/journal.pone.0199091

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