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microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb
Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. C...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002138/ https://www.ncbi.nlm.nih.gov/pubmed/29791595 http://dx.doi.org/10.1590/1414-431X20186452 |
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author | Yang, Shaofeng Sheng, Nan Pan, Lili Cao, Jing Liu, Jiao Ma, Ran |
author_facet | Yang, Shaofeng Sheng, Nan Pan, Lili Cao, Jing Liu, Jiao Ma, Ran |
author_sort | Yang, Shaofeng |
collection | PubMed |
description | Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC. |
format | Online Article Text |
id | pubmed-6002138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-60021382018-07-06 microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb Yang, Shaofeng Sheng, Nan Pan, Lili Cao, Jing Liu, Jiao Ma, Ran Braz J Med Biol Res Research Articles Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC. Associação Brasileira de Divulgação Científica 2018-05-21 /pmc/articles/PMC6002138/ /pubmed/29791595 http://dx.doi.org/10.1590/1414-431X20186452 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Yang, Shaofeng Sheng, Nan Pan, Lili Cao, Jing Liu, Jiao Ma, Ran microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb |
title | microRNA-3129 promotes cell proliferation in gastric cancer cell line
SGC7901 via positive regulation of pRb |
title_full | microRNA-3129 promotes cell proliferation in gastric cancer cell line
SGC7901 via positive regulation of pRb |
title_fullStr | microRNA-3129 promotes cell proliferation in gastric cancer cell line
SGC7901 via positive regulation of pRb |
title_full_unstemmed | microRNA-3129 promotes cell proliferation in gastric cancer cell line
SGC7901 via positive regulation of pRb |
title_short | microRNA-3129 promotes cell proliferation in gastric cancer cell line
SGC7901 via positive regulation of pRb |
title_sort | microrna-3129 promotes cell proliferation in gastric cancer cell line
sgc7901 via positive regulation of prb |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002138/ https://www.ncbi.nlm.nih.gov/pubmed/29791595 http://dx.doi.org/10.1590/1414-431X20186452 |
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