Cargando…

Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols

Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zik...

Descripción completa

Detalles Bibliográficos
Autores principales:	de Moraes, F.M., Espósito, D.L.A., Klein, T.M., da Fonseca, B.A.L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Associação Brasileira de Divulgação Científica 2018
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002146/ https://www.ncbi.nlm.nih.gov/pubmed/29791591 http://dx.doi.org/10.1590/1414-431X20187221

Ejemplares similares

Multiplex real time PCRs for respiratory viral infections
por: Nielsen, L.P., et al.
Publicado: (2006)

Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
por: Jalal, Sehrish, et al.
Publicado: (2021)

Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
por: Emmler, Laura, et al.
Publicado: (2019)

Comparison of Pan-Lyssavirus RT-PCRs and Development of an Improved Protocol for Surveillance of Non-RABV Lyssaviruses
por: Drzewnioková, Petra, et al.
Publicado: (2023)

New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
por: Blanda, Valeria, et al.
Publicado: (2020)

Rapid detection and subtyping of European swine influenza viruses in porcine clinical samples by haemagglutinin‐ and neuraminidase‐specific tetra‐ and triplex real‐time RT‐PCRs
por: Henritzi, Dinah, et al.
Publicado: (2016)

Multiplex real time PCRs for detection of influenza A subtypes including H5N1
por: Nielsen, L.P., et al.
Publicado: (2006)

Prevalence and detection of Tropheryma whipplei in the stools of Korean patients with diarrhea using real-time PCRs
por: Yoon, Sumi, et al.
Publicado: (2022)

Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
por: Santiago, Gilberto A., et al.
Publicado: (2018)

Comparative Evaluation of Three Commercial Quantitative Real-Time PCRs Used in Japan for Bovine Leukemia Virus
por: Yoneyama, Syuji, et al.
Publicado: (2022)

New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
por: Naguib, Mahmoud M., et al.
Publicado: (2017)

Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs
por: Maksimov, Pavlo, et al.
Publicado: (2020)

Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples
por: Tanida, Konstantin, et al.
Publicado: (2021)

Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks
por: Yang, Jifei, et al.
Publicado: (2016)

High-throughput multiplex qPCRs for the surveillance of zoonotic species of canine hookworms
por: Massetti, Luca, et al.
Publicado: (2020)

A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs
por: Wu, Di, et al.
Publicado: (2019)

Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection of Schistosoma japonicum Complex DNA in Human Serum
por: Frickmann, Hagen, et al.
Publicado: (2021)

Allele-Specific Dual PCRs to Identify Members of the 27a Cluster of PPV
por: Tamás, Vivien, et al.
Publicado: (2022)

Vaccination against the Protozoan Parasite Histomonas meleagridis Primes the Activation of Toll-like Receptors in Turkeys and Chickens Determined by a Set of Newly Developed Multiplex RT-qPCRs
por: Mitra, Taniya, et al.
Publicado: (2021)

Detection of Listeria Spp. and Listeria Monocytogenes in Biological Samples by SYBR Green I and TaqMan Probe-based Real-time PCRs
por: Kędrak-Jabłońska, Agnieszka, et al.
Publicado: (2017)

Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples
por: Hoffmann, Tanja, et al.
Publicado: (2021)

Performance Characteristics of Real-Time PCRs for African Swine Fever Virus Genome Detection—Comparison of Twelve Kits to an OIE-Recommended Method
por: Pikalo, Jutta, et al.
Publicado: (2022)

Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.
por: Chicharro, Carmen, et al.
Publicado: (2023)

Comparison of MY09/11 consensus PCR and type-specific PCRs in the detection of oncogenic HPV types
por: Depuydt, C E, et al.
Publicado: (2007)

Development of new host‐specific Bacteroides qPCRs for the identification of fecal contamination sources in water
por: Gómez‐Doñate, Marta, et al.
Publicado: (2016)

Adjuvant immunotherapy in resected esophageal squamous cell carcinoma: a gospel to the non-pCRs
por: Zhang, Zhihui, et al.
Publicado: (2021)

Controversial Opinion of “All pCRs are the Same” in St. Gallen International Consensus Guidelines 2021
por: Cong, Bin-Bin, et al.
Publicado: (2022)

The Prospective Co-Parenting Relationship Scale (PCRS) for Sexual Minority and Heterosexual People: Preliminary Validation
por: Leal, Daniela, et al.
Publicado: (2022)

Outpatient palliative care referral system (PCRS) for patients with advanced cancer: an impact evaluation protocol
por: Brunelli, Cinzia, et al.
Publicado: (2022)

Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?
por: Babouee Flury, B, et al.
Publicado: (2014)

Real-Time RT-PCR for the Detection of Lyssavirus Species
por: Deubelbeiss, A., et al.
Publicado: (2014)

PERFORMANCE OF CONVENTIONAL PCRs BASED ON PRIMERS DIRECTED TO NUCLEAR AND MITOCHONDRIAL GENES FOR THE DETECTION AND IDENTIFICATION OF Leishmania spp.
por: LOPES, Estela Gallucci, et al.
Publicado: (2016)

Selected abstracts from the PCRS-UK National Primary Care Respiratory Conference, Telford, October 2013
Publicado: (2013)

Abstract 109: Long-range PCRs and MLPA for molecular analysis of large rearrangements in 21 hydroxylase deficiency
por: Ravichandran, Lavanya, et al.
Publicado: (2022)

Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific
por: Huggins, Lucas, et al.
Publicado: (2021)

Partner Referral by HIV-Infected Persons to Partner Counseling and Referral Services (PCRS) - Results from a Demonstration Project
por: Song, Binwei, et al.
Publicado: (2012)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Zika virus infections imported from Brazil to Portugal, 2015
por: Zé-Zé, L., et al.
Publicado: (2016)

Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China
por: Cai, Cheng-Song, et al.
Publicado: (2010)

A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital
por: Hardegen, Christoph, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_ahti9c6p72j7fkoajne6rp2fev