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Transcript variants and expression profiles analysis of Mitf gene in minipigs
OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chinese PLA General Hospital
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002570/ https://www.ncbi.nlm.nih.gov/pubmed/29937787 http://dx.doi.org/10.1016/j.joto.2015.09.005 |
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author | Guo, Weiwei Ren, Lili Chen, Lei Ning, Yu Zhao, Lidong Yang, Shiming |
author_facet | Guo, Weiwei Ren, Lili Chen, Lei Ning, Yu Zhao, Lidong Yang, Shiming |
author_sort | Guo, Weiwei |
collection | PubMed |
description | OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. RESULTS: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. CONCLUSION: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF. |
format | Online Article Text |
id | pubmed-6002570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Chinese PLA General Hospital |
record_format | MEDLINE/PubMed |
spelling | pubmed-60025702018-06-22 Transcript variants and expression profiles analysis of Mitf gene in minipigs Guo, Weiwei Ren, Lili Chen, Lei Ning, Yu Zhao, Lidong Yang, Shiming J Otol Original article OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. RESULTS: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. CONCLUSION: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF. Chinese PLA General Hospital 2015-06 2015-09-30 /pmc/articles/PMC6002570/ /pubmed/29937787 http://dx.doi.org/10.1016/j.joto.2015.09.005 Text en © 2015 PLA General Hospital Department of Otolaryngology Head and Neck Surgery http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Guo, Weiwei Ren, Lili Chen, Lei Ning, Yu Zhao, Lidong Yang, Shiming Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title | Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title_full | Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title_fullStr | Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title_full_unstemmed | Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title_short | Transcript variants and expression profiles analysis of Mitf gene in minipigs |
title_sort | transcript variants and expression profiles analysis of mitf gene in minipigs |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002570/ https://www.ncbi.nlm.nih.gov/pubmed/29937787 http://dx.doi.org/10.1016/j.joto.2015.09.005 |
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