Cargando…

Transcript variants and expression profiles analysis of Mitf gene in minipigs

OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression p...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Weiwei, Ren, Lili, Chen, Lei, Ning, Yu, Zhao, Lidong, Yang, Shiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese PLA General Hospital 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002570/
https://www.ncbi.nlm.nih.gov/pubmed/29937787
http://dx.doi.org/10.1016/j.joto.2015.09.005
_version_ 1783332236688359424
author Guo, Weiwei
Ren, Lili
Chen, Lei
Ning, Yu
Zhao, Lidong
Yang, Shiming
author_facet Guo, Weiwei
Ren, Lili
Chen, Lei
Ning, Yu
Zhao, Lidong
Yang, Shiming
author_sort Guo, Weiwei
collection PubMed
description OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. RESULTS: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. CONCLUSION: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF.
format Online
Article
Text
id pubmed-6002570
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Chinese PLA General Hospital
record_format MEDLINE/PubMed
spelling pubmed-60025702018-06-22 Transcript variants and expression profiles analysis of Mitf gene in minipigs Guo, Weiwei Ren, Lili Chen, Lei Ning, Yu Zhao, Lidong Yang, Shiming J Otol Original article OBJECT: To identify transcript variants and expression patterns of porcine Mitf. MATERIALS AND METHODS: A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5′ RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. RESULTS: Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. CONCLUSION: The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF. Chinese PLA General Hospital 2015-06 2015-09-30 /pmc/articles/PMC6002570/ /pubmed/29937787 http://dx.doi.org/10.1016/j.joto.2015.09.005 Text en © 2015 PLA General Hospital Department of Otolaryngology Head and Neck Surgery http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Guo, Weiwei
Ren, Lili
Chen, Lei
Ning, Yu
Zhao, Lidong
Yang, Shiming
Transcript variants and expression profiles analysis of Mitf gene in minipigs
title Transcript variants and expression profiles analysis of Mitf gene in minipigs
title_full Transcript variants and expression profiles analysis of Mitf gene in minipigs
title_fullStr Transcript variants and expression profiles analysis of Mitf gene in minipigs
title_full_unstemmed Transcript variants and expression profiles analysis of Mitf gene in minipigs
title_short Transcript variants and expression profiles analysis of Mitf gene in minipigs
title_sort transcript variants and expression profiles analysis of mitf gene in minipigs
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6002570/
https://www.ncbi.nlm.nih.gov/pubmed/29937787
http://dx.doi.org/10.1016/j.joto.2015.09.005
work_keys_str_mv AT guoweiwei transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs
AT renlili transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs
AT chenlei transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs
AT ningyu transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs
AT zhaolidong transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs
AT yangshiming transcriptvariantsandexpressionprofilesanalysisofmitfgeneinminipigs