Cargando…
Over-expression of mammaglobin-B in canine mammary tumors
BACKGROUND: Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammagl...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003043/ https://www.ncbi.nlm.nih.gov/pubmed/29903015 http://dx.doi.org/10.1186/s12917-018-1507-z |
_version_ | 1783332295504035840 |
---|---|
author | Pandey, Mamta Sunil Kumar, B. V. Gupta, Kuldip Sethi, Ram Saran Kumar, Ashwani Verma, Ramneek |
author_facet | Pandey, Mamta Sunil Kumar, B. V. Gupta, Kuldip Sethi, Ram Saran Kumar, Ashwani Verma, Ramneek |
author_sort | Pandey, Mamta |
collection | PubMed |
description | BACKGROUND: Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammaglobin-B gene and protein in canine mammary tumor (CMT) tissue. Expression of mammaglobin-B mRNA and protein were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. RESULTS: High levels of mammaglobin-B mRNA expression (6.663 ± 0.841times) was observed in CMT as compared to age and breed matched healthy controls. Further, expression of mammaglobin-B protein was detected in paraffin-embedded mammary tumor tissues from the same subjects by IHC. Mammaglobin-B protein was overexpressed only in 6.67% of healthy mammary glands while, a high level of its expression was scored in 76.7% of the CMT subjects. Moreover, no significant differences in terms of IHC score and qRT-PCR score with respect to CMT histotypes or tumor grades were observed, indicating that mammaglobin-B over-expression occurred irrespective of CMT types or grades. CONCLUSION: Overall, significantly increased expression of mammaglobin-B protein was found in CMTs with respect to healthy mammary glands, which positively correlates to its transcript. These findings suggest that overexpression of mammaglobin-B is associated with tumors of canine mammary glands. |
format | Online Article Text |
id | pubmed-6003043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60030432018-07-06 Over-expression of mammaglobin-B in canine mammary tumors Pandey, Mamta Sunil Kumar, B. V. Gupta, Kuldip Sethi, Ram Saran Kumar, Ashwani Verma, Ramneek BMC Vet Res Research Article BACKGROUND: Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammaglobin-B gene and protein in canine mammary tumor (CMT) tissue. Expression of mammaglobin-B mRNA and protein were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. RESULTS: High levels of mammaglobin-B mRNA expression (6.663 ± 0.841times) was observed in CMT as compared to age and breed matched healthy controls. Further, expression of mammaglobin-B protein was detected in paraffin-embedded mammary tumor tissues from the same subjects by IHC. Mammaglobin-B protein was overexpressed only in 6.67% of healthy mammary glands while, a high level of its expression was scored in 76.7% of the CMT subjects. Moreover, no significant differences in terms of IHC score and qRT-PCR score with respect to CMT histotypes or tumor grades were observed, indicating that mammaglobin-B over-expression occurred irrespective of CMT types or grades. CONCLUSION: Overall, significantly increased expression of mammaglobin-B protein was found in CMTs with respect to healthy mammary glands, which positively correlates to its transcript. These findings suggest that overexpression of mammaglobin-B is associated with tumors of canine mammary glands. BioMed Central 2018-06-15 /pmc/articles/PMC6003043/ /pubmed/29903015 http://dx.doi.org/10.1186/s12917-018-1507-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Pandey, Mamta Sunil Kumar, B. V. Gupta, Kuldip Sethi, Ram Saran Kumar, Ashwani Verma, Ramneek Over-expression of mammaglobin-B in canine mammary tumors |
title | Over-expression of mammaglobin-B in canine mammary tumors |
title_full | Over-expression of mammaglobin-B in canine mammary tumors |
title_fullStr | Over-expression of mammaglobin-B in canine mammary tumors |
title_full_unstemmed | Over-expression of mammaglobin-B in canine mammary tumors |
title_short | Over-expression of mammaglobin-B in canine mammary tumors |
title_sort | over-expression of mammaglobin-b in canine mammary tumors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003043/ https://www.ncbi.nlm.nih.gov/pubmed/29903015 http://dx.doi.org/10.1186/s12917-018-1507-z |
work_keys_str_mv | AT pandeymamta overexpressionofmammaglobinbincaninemammarytumors AT sunilkumarbv overexpressionofmammaglobinbincaninemammarytumors AT guptakuldip overexpressionofmammaglobinbincaninemammarytumors AT sethiramsaran overexpressionofmammaglobinbincaninemammarytumors AT kumarashwani overexpressionofmammaglobinbincaninemammarytumors AT vermaramneek overexpressionofmammaglobinbincaninemammarytumors |