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Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response

Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pul...

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Autores principales: Tzeng, Stephany Y., McHugh, Kevin J., Behrens, Adam M., Rose, Sviatlana, Sugarman, James L., Ferber, Shiran, Langer, Robert, Jaklenec, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003376/
https://www.ncbi.nlm.nih.gov/pubmed/29784798
http://dx.doi.org/10.1073/pnas.1720970115
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author Tzeng, Stephany Y.
McHugh, Kevin J.
Behrens, Adam M.
Rose, Sviatlana
Sugarman, James L.
Ferber, Shiran
Langer, Robert
Jaklenec, Ana
author_facet Tzeng, Stephany Y.
McHugh, Kevin J.
Behrens, Adam M.
Rose, Sviatlana
Sugarman, James L.
Ferber, Shiran
Langer, Robert
Jaklenec, Ana
author_sort Tzeng, Stephany Y.
collection PubMed
description Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world.
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spelling pubmed-60033762018-06-18 Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response Tzeng, Stephany Y. McHugh, Kevin J. Behrens, Adam M. Rose, Sviatlana Sugarman, James L. Ferber, Shiran Langer, Robert Jaklenec, Ana Proc Natl Acad Sci U S A PNAS Plus Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world. National Academy of Sciences 2018-06-05 2018-05-21 /pmc/articles/PMC6003376/ /pubmed/29784798 http://dx.doi.org/10.1073/pnas.1720970115 Text en Copyright © 2018 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle PNAS Plus
Tzeng, Stephany Y.
McHugh, Kevin J.
Behrens, Adam M.
Rose, Sviatlana
Sugarman, James L.
Ferber, Shiran
Langer, Robert
Jaklenec, Ana
Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title_full Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title_fullStr Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title_full_unstemmed Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title_short Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
title_sort stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response
topic PNAS Plus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003376/
https://www.ncbi.nlm.nih.gov/pubmed/29784798
http://dx.doi.org/10.1073/pnas.1720970115
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