β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification

Ghrelin is a unique fatty acid-modified peptide hormone produced in the stomach and has important roles in energy homeostasis and gastrointestinal motility. However, the medium-chain fatty acid source for ghrelin acyl-modification is not known. We found that a fat-free diet and the removal of intest...

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Autores principales: Ikenoya, Chika, Takemi, Shota, Kaminoda, Arisa, Aizawa, Sayaka, Ojima, Shiomi, Gong, Zhi, Chacrabati, Rakhi, Kondo, Daisuke, Wada, Reiko, Tanaka, Toru, Tsuda, Sachiko, Sakai, Takafumi, Sakata, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003948/
https://www.ncbi.nlm.nih.gov/pubmed/29907775
http://dx.doi.org/10.1038/s41598-018-27458-2
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author Ikenoya, Chika
Takemi, Shota
Kaminoda, Arisa
Aizawa, Sayaka
Ojima, Shiomi
Gong, Zhi
Chacrabati, Rakhi
Kondo, Daisuke
Wada, Reiko
Tanaka, Toru
Tsuda, Sachiko
Sakai, Takafumi
Sakata, Ichiro
author_facet Ikenoya, Chika
Takemi, Shota
Kaminoda, Arisa
Aizawa, Sayaka
Ojima, Shiomi
Gong, Zhi
Chacrabati, Rakhi
Kondo, Daisuke
Wada, Reiko
Tanaka, Toru
Tsuda, Sachiko
Sakai, Takafumi
Sakata, Ichiro
author_sort Ikenoya, Chika
collection PubMed
description Ghrelin is a unique fatty acid-modified peptide hormone produced in the stomach and has important roles in energy homeostasis and gastrointestinal motility. However, the medium-chain fatty acid source for ghrelin acyl-modification is not known. We found that a fat-free diet and the removal of intestinal microbiota did not decrease acyl-ghrelin production in the stomach or plasma acyl-ghrelin levels in mice. RT-PCR analysis showed that genes involving fatty acid synthesis, metabolism, and transport were expressed in pancreas-derived ghrelinoma (PG-1) cells. Treatment with an irreversible inhibitor of carnitine palmitoyltransferase-1 (CPT-1) strongly decreased acylated ghrelin levels but did not affect ghrelin or ghrelin o-acyl transferase (GOAT) mRNA levels in PG-1 cells. Our results suggest that the medium-chain fatty acid used for the acyl-modification of ghrelin is produced in ghrelin-producing cells themselves by β-oxidation of long-chain fatty acids provided from the circulation.
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spelling pubmed-60039482018-06-26 β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification Ikenoya, Chika Takemi, Shota Kaminoda, Arisa Aizawa, Sayaka Ojima, Shiomi Gong, Zhi Chacrabati, Rakhi Kondo, Daisuke Wada, Reiko Tanaka, Toru Tsuda, Sachiko Sakai, Takafumi Sakata, Ichiro Sci Rep Article Ghrelin is a unique fatty acid-modified peptide hormone produced in the stomach and has important roles in energy homeostasis and gastrointestinal motility. However, the medium-chain fatty acid source for ghrelin acyl-modification is not known. We found that a fat-free diet and the removal of intestinal microbiota did not decrease acyl-ghrelin production in the stomach or plasma acyl-ghrelin levels in mice. RT-PCR analysis showed that genes involving fatty acid synthesis, metabolism, and transport were expressed in pancreas-derived ghrelinoma (PG-1) cells. Treatment with an irreversible inhibitor of carnitine palmitoyltransferase-1 (CPT-1) strongly decreased acylated ghrelin levels but did not affect ghrelin or ghrelin o-acyl transferase (GOAT) mRNA levels in PG-1 cells. Our results suggest that the medium-chain fatty acid used for the acyl-modification of ghrelin is produced in ghrelin-producing cells themselves by β-oxidation of long-chain fatty acids provided from the circulation. Nature Publishing Group UK 2018-06-15 /pmc/articles/PMC6003948/ /pubmed/29907775 http://dx.doi.org/10.1038/s41598-018-27458-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ikenoya, Chika
Takemi, Shota
Kaminoda, Arisa
Aizawa, Sayaka
Ojima, Shiomi
Gong, Zhi
Chacrabati, Rakhi
Kondo, Daisuke
Wada, Reiko
Tanaka, Toru
Tsuda, Sachiko
Sakai, Takafumi
Sakata, Ichiro
β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title_full β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title_fullStr β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title_full_unstemmed β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title_short β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
title_sort β-oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003948/
https://www.ncbi.nlm.nih.gov/pubmed/29907775
http://dx.doi.org/10.1038/s41598-018-27458-2
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