Cargando…
Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides
Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of the host. The detectability by carbohydrate-binding proteins of glycan antigens and ligand...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003998/ https://www.ncbi.nlm.nih.gov/pubmed/29752599 http://dx.doi.org/10.1007/s13361-018-1944-8 |
_version_ | 1783332443957231616 |
---|---|
author | Chai, Wengang Zhang, Yibing Mauri, Laura Ciampa, Maria G. Mulloy, Barbara Sonnino, Sandro Feizi, Ten |
author_facet | Chai, Wengang Zhang, Yibing Mauri, Laura Ciampa, Maria G. Mulloy, Barbara Sonnino, Sandro Feizi, Ten |
author_sort | Chai, Wengang |
collection | PubMed |
description | Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of the host. The detectability by carbohydrate-binding proteins of glycan antigens and ligands on glycolipids can be influenced by the differing lipid moieties. To investigate glycan sequences of gangliosides as recognition structures, we have underway a program of work to develop a “gangliome” microarray consisting of isolated natural gangliosides and neoglycolipids (NGLs) derived from glycans released from them, and each linked to the same lipid molecule for arraying and comparative microarray binding analyses. Here, in the first phase of our studies, we describe a strategy for high-sensitivity assignment of the tetrasaccharide backbones and application to identification of eight of monosialylated glycans released from bovine brain gangliosides. This approach is based on negative-ion electrospray mass spectrometry with collision-induced dissociation (ESI-CID-MS/MS) of the desialylated glycans. Using this strategy, we have the data on backbone regions of four minor components among the monosialo-ganglioside-derived glycans; these are of the ganglio-, lacto-, and neolacto-series. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-018-1944-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6003998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-60039982018-07-02 Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides Chai, Wengang Zhang, Yibing Mauri, Laura Ciampa, Maria G. Mulloy, Barbara Sonnino, Sandro Feizi, Ten J Am Soc Mass Spectrom Focus: Mass Spectrometry in Glycobiology and Related Fields: Research Article Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of the host. The detectability by carbohydrate-binding proteins of glycan antigens and ligands on glycolipids can be influenced by the differing lipid moieties. To investigate glycan sequences of gangliosides as recognition structures, we have underway a program of work to develop a “gangliome” microarray consisting of isolated natural gangliosides and neoglycolipids (NGLs) derived from glycans released from them, and each linked to the same lipid molecule for arraying and comparative microarray binding analyses. Here, in the first phase of our studies, we describe a strategy for high-sensitivity assignment of the tetrasaccharide backbones and application to identification of eight of monosialylated glycans released from bovine brain gangliosides. This approach is based on negative-ion electrospray mass spectrometry with collision-induced dissociation (ESI-CID-MS/MS) of the desialylated glycans. Using this strategy, we have the data on backbone regions of four minor components among the monosialo-ganglioside-derived glycans; these are of the ganglio-, lacto-, and neolacto-series. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-018-1944-8) contains supplementary material, which is available to authorized users. Springer US 2018-05-11 2018 /pmc/articles/PMC6003998/ /pubmed/29752599 http://dx.doi.org/10.1007/s13361-018-1944-8 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Focus: Mass Spectrometry in Glycobiology and Related Fields: Research Article Chai, Wengang Zhang, Yibing Mauri, Laura Ciampa, Maria G. Mulloy, Barbara Sonnino, Sandro Feizi, Ten Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title | Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title_full | Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title_fullStr | Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title_full_unstemmed | Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title_short | Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides |
title_sort | assignment by negative-ion electrospray tandem mass spectrometry of the tetrasaccharide backbones of monosialylated glycans released from bovine brain gangliosides |
topic | Focus: Mass Spectrometry in Glycobiology and Related Fields: Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003998/ https://www.ncbi.nlm.nih.gov/pubmed/29752599 http://dx.doi.org/10.1007/s13361-018-1944-8 |
work_keys_str_mv | AT chaiwengang assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT zhangyibing assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT maurilaura assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT ciampamariag assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT mulloybarbara assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT sonninosandro assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides AT feiziten assignmentbynegativeionelectrospraytandemmassspectrometryofthetetrasaccharidebackbonesofmonosialylatedglycansreleasedfrombovinebraingangliosides |