In planta proximity dependent biotin identification (BioID)

Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate...

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Autores principales: Khan, Madiha, Youn, Ji-Young, Gingras, Anne-Claude, Subramaniam, Rajagopal, Desveaux, Darrell
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004002/
https://www.ncbi.nlm.nih.gov/pubmed/29907827
http://dx.doi.org/10.1038/s41598-018-27500-3
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author Khan, Madiha
Youn, Ji-Young
Gingras, Anne-Claude
Subramaniam, Rajagopal
Desveaux, Darrell
author_facet Khan, Madiha
Youn, Ji-Young
Gingras, Anne-Claude
Subramaniam, Rajagopal
Desveaux, Darrell
author_sort Khan, Madiha
collection PubMed
description Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
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spelling pubmed-60040022018-06-26 In planta proximity dependent biotin identification (BioID) Khan, Madiha Youn, Ji-Young Gingras, Anne-Claude Subramaniam, Rajagopal Desveaux, Darrell Sci Rep Article Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists. Nature Publishing Group UK 2018-06-15 /pmc/articles/PMC6004002/ /pubmed/29907827 http://dx.doi.org/10.1038/s41598-018-27500-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Khan, Madiha
Youn, Ji-Young
Gingras, Anne-Claude
Subramaniam, Rajagopal
Desveaux, Darrell
In planta proximity dependent biotin identification (BioID)
title In planta proximity dependent biotin identification (BioID)
title_full In planta proximity dependent biotin identification (BioID)
title_fullStr In planta proximity dependent biotin identification (BioID)
title_full_unstemmed In planta proximity dependent biotin identification (BioID)
title_short In planta proximity dependent biotin identification (BioID)
title_sort in planta proximity dependent biotin identification (bioid)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004002/
https://www.ncbi.nlm.nih.gov/pubmed/29907827
http://dx.doi.org/10.1038/s41598-018-27500-3
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