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Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line
Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the “leading circle” and the “sp...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004038/ https://www.ncbi.nlm.nih.gov/pubmed/29746849 http://dx.doi.org/10.1016/j.yjmcc.2018.05.002 |
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author | Houston, Charles Tzortzis, Konstantinos N. Roney, Caroline Saglietto, Andrea Pitcher, David S. Cantwell, Chris D. Chowdhury, Rasheda A. Ng, Fu Siong Peters, Nicholas S. Dupont, Emmanuel |
author_facet | Houston, Charles Tzortzis, Konstantinos N. Roney, Caroline Saglietto, Andrea Pitcher, David S. Cantwell, Chris D. Chowdhury, Rasheda A. Ng, Fu Siong Peters, Nicholas S. Dupont, Emmanuel |
author_sort | Houston, Charles |
collection | PubMed |
description | Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the “leading circle” and the “spiral wave or rotor” theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level. We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entry. Re-entries in HL1-6 myocytes required at least two triggers and a minimum colony area to initiate (3.5 to 6.4 mm(2)). After electrical activity was completely stopped and re-started by varying the extracellular K(+) concentration, re-entries never returned to the same location while 35% of triggers re-appeared at the same position. A conduction delay algorithm also allows visualisation of the core of the re-entries. This work has revealed that the core of re-entries is conduction blocks constituted by lines and/or groups of cells rather than the round area assumed by the other concepts of functional re-entry. This highlights the importance of experimentation at the microscopic level in the study of re-entry mechanisms. |
format | Online Article Text |
id | pubmed-6004038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60040382018-06-18 Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line Houston, Charles Tzortzis, Konstantinos N. Roney, Caroline Saglietto, Andrea Pitcher, David S. Cantwell, Chris D. Chowdhury, Rasheda A. Ng, Fu Siong Peters, Nicholas S. Dupont, Emmanuel J Mol Cell Cardiol Article Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the “leading circle” and the “spiral wave or rotor” theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level. We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entry. Re-entries in HL1-6 myocytes required at least two triggers and a minimum colony area to initiate (3.5 to 6.4 mm(2)). After electrical activity was completely stopped and re-started by varying the extracellular K(+) concentration, re-entries never returned to the same location while 35% of triggers re-appeared at the same position. A conduction delay algorithm also allows visualisation of the core of the re-entries. This work has revealed that the core of re-entries is conduction blocks constituted by lines and/or groups of cells rather than the round area assumed by the other concepts of functional re-entry. This highlights the importance of experimentation at the microscopic level in the study of re-entry mechanisms. Academic Press 2018-06 /pmc/articles/PMC6004038/ /pubmed/29746849 http://dx.doi.org/10.1016/j.yjmcc.2018.05.002 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Houston, Charles Tzortzis, Konstantinos N. Roney, Caroline Saglietto, Andrea Pitcher, David S. Cantwell, Chris D. Chowdhury, Rasheda A. Ng, Fu Siong Peters, Nicholas S. Dupont, Emmanuel Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title | Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title_full | Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title_fullStr | Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title_full_unstemmed | Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title_short | Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line |
title_sort | characterisation of re-entrant circuit (or rotational activity) in vitro using the hl1-6 myocyte cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004038/ https://www.ncbi.nlm.nih.gov/pubmed/29746849 http://dx.doi.org/10.1016/j.yjmcc.2018.05.002 |
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