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7SL RNA in vertebrate red blood cells
We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004055/ https://www.ncbi.nlm.nih.gov/pubmed/29686135 http://dx.doi.org/10.1261/rna.065474.117 |
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author | Talhouarne, Gaëlle J.S. Gall, Joseph G. |
author_facet | Talhouarne, Gaëlle J.S. Gall, Joseph G. |
author_sort | Talhouarne, Gaëlle J.S. |
collection | PubMed |
description | We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from a lysate of RBCs with a number of membrane-binding proteins. Human and mouse RBCs also contain a previously undescribed 68 nt RNA, sRN7SL, derived from the “S domain” of 7SL RNA. We discuss the possibility that 7SL RNA is selectively protected from nucleases by association with the RBC membrane. Because 7SL is not associated with the canonical proteins of the SRP, it could represent a nonfunctional remnant of the protein synthetic machinery. Alternatively, it could play a new, as yet undefined role in RBC metabolism. |
format | Online Article Text |
id | pubmed-6004055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60040552018-07-01 7SL RNA in vertebrate red blood cells Talhouarne, Gaëlle J.S. Gall, Joseph G. RNA Report We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from a lysate of RBCs with a number of membrane-binding proteins. Human and mouse RBCs also contain a previously undescribed 68 nt RNA, sRN7SL, derived from the “S domain” of 7SL RNA. We discuss the possibility that 7SL RNA is selectively protected from nucleases by association with the RBC membrane. Because 7SL is not associated with the canonical proteins of the SRP, it could represent a nonfunctional remnant of the protein synthetic machinery. Alternatively, it could play a new, as yet undefined role in RBC metabolism. Cold Spring Harbor Laboratory Press 2018-07 /pmc/articles/PMC6004055/ /pubmed/29686135 http://dx.doi.org/10.1261/rna.065474.117 Text en © 2018 Talhouarne and Gall; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Report Talhouarne, Gaëlle J.S. Gall, Joseph G. 7SL RNA in vertebrate red blood cells |
title | 7SL RNA in vertebrate red blood cells |
title_full | 7SL RNA in vertebrate red blood cells |
title_fullStr | 7SL RNA in vertebrate red blood cells |
title_full_unstemmed | 7SL RNA in vertebrate red blood cells |
title_short | 7SL RNA in vertebrate red blood cells |
title_sort | 7sl rna in vertebrate red blood cells |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004055/ https://www.ncbi.nlm.nih.gov/pubmed/29686135 http://dx.doi.org/10.1261/rna.065474.117 |
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