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7SL RNA in vertebrate red blood cells

We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from...

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Detalles Bibliográficos
Autores principales: Talhouarne, Gaëlle J.S., Gall, Joseph G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004055/
https://www.ncbi.nlm.nih.gov/pubmed/29686135
http://dx.doi.org/10.1261/rna.065474.117
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author Talhouarne, Gaëlle J.S.
Gall, Joseph G.
author_facet Talhouarne, Gaëlle J.S.
Gall, Joseph G.
author_sort Talhouarne, Gaëlle J.S.
collection PubMed
description We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from a lysate of RBCs with a number of membrane-binding proteins. Human and mouse RBCs also contain a previously undescribed 68 nt RNA, sRN7SL, derived from the “S domain” of 7SL RNA. We discuss the possibility that 7SL RNA is selectively protected from nucleases by association with the RBC membrane. Because 7SL is not associated with the canonical proteins of the SRP, it could represent a nonfunctional remnant of the protein synthetic machinery. Alternatively, it could play a new, as yet undefined role in RBC metabolism.
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spelling pubmed-60040552018-07-01 7SL RNA in vertebrate red blood cells Talhouarne, Gaëlle J.S. Gall, Joseph G. RNA Report We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from a lysate of RBCs with a number of membrane-binding proteins. Human and mouse RBCs also contain a previously undescribed 68 nt RNA, sRN7SL, derived from the “S domain” of 7SL RNA. We discuss the possibility that 7SL RNA is selectively protected from nucleases by association with the RBC membrane. Because 7SL is not associated with the canonical proteins of the SRP, it could represent a nonfunctional remnant of the protein synthetic machinery. Alternatively, it could play a new, as yet undefined role in RBC metabolism. Cold Spring Harbor Laboratory Press 2018-07 /pmc/articles/PMC6004055/ /pubmed/29686135 http://dx.doi.org/10.1261/rna.065474.117 Text en © 2018 Talhouarne and Gall; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Report
Talhouarne, Gaëlle J.S.
Gall, Joseph G.
7SL RNA in vertebrate red blood cells
title 7SL RNA in vertebrate red blood cells
title_full 7SL RNA in vertebrate red blood cells
title_fullStr 7SL RNA in vertebrate red blood cells
title_full_unstemmed 7SL RNA in vertebrate red blood cells
title_short 7SL RNA in vertebrate red blood cells
title_sort 7sl rna in vertebrate red blood cells
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004055/
https://www.ncbi.nlm.nih.gov/pubmed/29686135
http://dx.doi.org/10.1261/rna.065474.117
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