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Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters

RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and “proximal sequence element” (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the...

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Autores principales: Dergai, Oleksandr, Cousin, Pascal, Gouge, Jerome, Satia, Karishma, Praz, Viviane, Kuhlman, Tracy, Lhôte, Philippe, Vannini, Alessandro, Hernandez, Nouria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004067/
https://www.ncbi.nlm.nih.gov/pubmed/29785964
http://dx.doi.org/10.1101/gad.314245.118
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author Dergai, Oleksandr
Cousin, Pascal
Gouge, Jerome
Satia, Karishma
Praz, Viviane
Kuhlman, Tracy
Lhôte, Philippe
Vannini, Alessandro
Hernandez, Nouria
author_facet Dergai, Oleksandr
Cousin, Pascal
Gouge, Jerome
Satia, Karishma
Praz, Viviane
Kuhlman, Tracy
Lhôte, Philippe
Vannini, Alessandro
Hernandez, Nouria
author_sort Dergai, Oleksandr
collection PubMed
description RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and “proximal sequence element” (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP–TFIIB and TBP–BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP–BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
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spelling pubmed-60040672018-06-29 Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters Dergai, Oleksandr Cousin, Pascal Gouge, Jerome Satia, Karishma Praz, Viviane Kuhlman, Tracy Lhôte, Philippe Vannini, Alessandro Hernandez, Nouria Genes Dev Research Paper RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and “proximal sequence element” (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP–TFIIB and TBP–BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP–BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters. Cold Spring Harbor Laboratory Press 2018-05-01 /pmc/articles/PMC6004067/ /pubmed/29785964 http://dx.doi.org/10.1101/gad.314245.118 Text en © 2018 Dergai et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Paper
Dergai, Oleksandr
Cousin, Pascal
Gouge, Jerome
Satia, Karishma
Praz, Viviane
Kuhlman, Tracy
Lhôte, Philippe
Vannini, Alessandro
Hernandez, Nouria
Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title_full Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title_fullStr Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title_full_unstemmed Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title_short Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
title_sort mechanism of selective recruitment of rna polymerases ii and iii to snrna gene promoters
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004067/
https://www.ncbi.nlm.nih.gov/pubmed/29785964
http://dx.doi.org/10.1101/gad.314245.118
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