A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs

Circular RNAs (circRNAs) are generated from many protein-coding genes. Most accumulate in the cytoplasm, but how circRNA localization or nuclear export is controlled remains unclear. Using RNAi screening, we found that depletion of the Drosophila DExH/D-box helicase Hel25E results in nuclear accumul...

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Autores principales: Huang, Chuan, Liang, Dongming, Tatomer, Deirdre C., Wilusz, Jeremy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004072/
https://www.ncbi.nlm.nih.gov/pubmed/29773557
http://dx.doi.org/10.1101/gad.314856.118
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author Huang, Chuan
Liang, Dongming
Tatomer, Deirdre C.
Wilusz, Jeremy E.
author_facet Huang, Chuan
Liang, Dongming
Tatomer, Deirdre C.
Wilusz, Jeremy E.
author_sort Huang, Chuan
collection PubMed
description Circular RNAs (circRNAs) are generated from many protein-coding genes. Most accumulate in the cytoplasm, but how circRNA localization or nuclear export is controlled remains unclear. Using RNAi screening, we found that depletion of the Drosophila DExH/D-box helicase Hel25E results in nuclear accumulation of long (>800-nucleotide), but not short, circRNAs. The human homologs of Hel25E similarly regulate circRNA localization, as depletion of UAP56 (DDX39B) or URH49 (DDX39A) causes long and short circRNAs, respectively, to become enriched in the nucleus. These data suggest that the lengths of mature circRNAs are measured to dictate the mode of nuclear export.
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spelling pubmed-60040722018-11-01 A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs Huang, Chuan Liang, Dongming Tatomer, Deirdre C. Wilusz, Jeremy E. Genes Dev Research Communication Circular RNAs (circRNAs) are generated from many protein-coding genes. Most accumulate in the cytoplasm, but how circRNA localization or nuclear export is controlled remains unclear. Using RNAi screening, we found that depletion of the Drosophila DExH/D-box helicase Hel25E results in nuclear accumulation of long (>800-nucleotide), but not short, circRNAs. The human homologs of Hel25E similarly regulate circRNA localization, as depletion of UAP56 (DDX39B) or URH49 (DDX39A) causes long and short circRNAs, respectively, to become enriched in the nucleus. These data suggest that the lengths of mature circRNAs are measured to dictate the mode of nuclear export. Cold Spring Harbor Laboratory Press 2018-05-01 /pmc/articles/PMC6004072/ /pubmed/29773557 http://dx.doi.org/10.1101/gad.314856.118 Text en © 2018 Huang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Huang, Chuan
Liang, Dongming
Tatomer, Deirdre C.
Wilusz, Jeremy E.
A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title_full A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title_fullStr A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title_full_unstemmed A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title_short A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs
title_sort length-dependent evolutionarily conserved pathway controls nuclear export of circular rnas
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004072/
https://www.ncbi.nlm.nih.gov/pubmed/29773557
http://dx.doi.org/10.1101/gad.314856.118
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