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Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway

BACKGROUND: Ubiquilin-4 (UBQLN4) is a component of the ubiquitin-proteasome system and regulates the degradation of many proteins implicated in pathological conditions. The aim of this study was to determine the role of UBQLN4 in regulating the proliferation and survival of the normal gastric epithe...

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Autores principales: Huang, Shengkai, Dong, Xin, Wang, Jia, Ding, Jie, Li, Yan, Li, Dongdong, Lin, Hong, Wang, Wenjie, Zhao, Mei, Chang, Qing, Zhou, Ning, Cui, Wei, Huang, Changzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004079/
https://www.ncbi.nlm.nih.gov/pubmed/29807370
http://dx.doi.org/10.12659/MSM.909621
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author Huang, Shengkai
Dong, Xin
Wang, Jia
Ding, Jie
Li, Yan
Li, Dongdong
Lin, Hong
Wang, Wenjie
Zhao, Mei
Chang, Qing
Zhou, Ning
Cui, Wei
Huang, Changzhi
author_facet Huang, Shengkai
Dong, Xin
Wang, Jia
Ding, Jie
Li, Yan
Li, Dongdong
Lin, Hong
Wang, Wenjie
Zhao, Mei
Chang, Qing
Zhou, Ning
Cui, Wei
Huang, Changzhi
author_sort Huang, Shengkai
collection PubMed
description BACKGROUND: Ubiquilin-4 (UBQLN4) is a component of the ubiquitin-proteasome system and regulates the degradation of many proteins implicated in pathological conditions. The aim of this study was to determine the role of UBQLN4 in regulating the proliferation and survival of the normal gastric epithelial cell line GES-1. MATERIAL/METHODS: We constructed GES-1 lines stably overexpressing UBQLN4 by lentiviral infection. Cell proliferation, apoptosis, and the cell cycle were analyzed using the MTT assay and flow cytometric assays. Phosphorylation of ERK, JNK, p38, and expression of cyclin D1 were detected by western blot analysis. RESULTS: Overexpression of UBQLN4 significantly reduced proliferation and induced G2/M phase arrest and apoptosis in GES-1 cells. Moreover, upregulation of UBQLN4 increased the expression of cyclin D1 and phosphorylated ERK, but not JNK or p38. CONCLUSIONS: These data suggest that UBQLN4 may induce cell cycle arrest and apoptosis via activation of the ERK pathway and upregulation of cyclin D1 in GES-1 cells.
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spelling pubmed-60040792018-06-18 Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway Huang, Shengkai Dong, Xin Wang, Jia Ding, Jie Li, Yan Li, Dongdong Lin, Hong Wang, Wenjie Zhao, Mei Chang, Qing Zhou, Ning Cui, Wei Huang, Changzhi Med Sci Monit Lab/In Vitro Research BACKGROUND: Ubiquilin-4 (UBQLN4) is a component of the ubiquitin-proteasome system and regulates the degradation of many proteins implicated in pathological conditions. The aim of this study was to determine the role of UBQLN4 in regulating the proliferation and survival of the normal gastric epithelial cell line GES-1. MATERIAL/METHODS: We constructed GES-1 lines stably overexpressing UBQLN4 by lentiviral infection. Cell proliferation, apoptosis, and the cell cycle were analyzed using the MTT assay and flow cytometric assays. Phosphorylation of ERK, JNK, p38, and expression of cyclin D1 were detected by western blot analysis. RESULTS: Overexpression of UBQLN4 significantly reduced proliferation and induced G2/M phase arrest and apoptosis in GES-1 cells. Moreover, upregulation of UBQLN4 increased the expression of cyclin D1 and phosphorylated ERK, but not JNK or p38. CONCLUSIONS: These data suggest that UBQLN4 may induce cell cycle arrest and apoptosis via activation of the ERK pathway and upregulation of cyclin D1 in GES-1 cells. International Scientific Literature, Inc. 2018-05-28 /pmc/articles/PMC6004079/ /pubmed/29807370 http://dx.doi.org/10.12659/MSM.909621 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Huang, Shengkai
Dong, Xin
Wang, Jia
Ding, Jie
Li, Yan
Li, Dongdong
Lin, Hong
Wang, Wenjie
Zhao, Mei
Chang, Qing
Zhou, Ning
Cui, Wei
Huang, Changzhi
Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title_full Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title_fullStr Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title_full_unstemmed Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title_short Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway
title_sort overexpression of the ubiquilin-4 (ubqln4) is associated with cell cycle arrest and apoptosis in human normal gastric epithelial cell lines ges-1 cells by activation of the erk signaling pathway
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004079/
https://www.ncbi.nlm.nih.gov/pubmed/29807370
http://dx.doi.org/10.12659/MSM.909621
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