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Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora

The goal of this research is the investigation of a way to maximize the production of docosahexaenoic acid (DHA) and β-carotene by optimizing the culture conditions of their sources, microalgae Schizochytrium limacinum and fungus Blakeslea trispora respectively, in a fermentation medium. The influen...

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Autores principales: Bindea, Maria, Rusu, Bogdan, Rusu, Alexandru, Trif, Monica, Leopold, Loredana Florina, Dulf, Francisc, Vodnar, Dan Cristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004094/
https://www.ncbi.nlm.nih.gov/pubmed/29908562
http://dx.doi.org/10.1186/s12934-018-0945-4
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author Bindea, Maria
Rusu, Bogdan
Rusu, Alexandru
Trif, Monica
Leopold, Loredana Florina
Dulf, Francisc
Vodnar, Dan Cristian
author_facet Bindea, Maria
Rusu, Bogdan
Rusu, Alexandru
Trif, Monica
Leopold, Loredana Florina
Dulf, Francisc
Vodnar, Dan Cristian
author_sort Bindea, Maria
collection PubMed
description The goal of this research is the investigation of a way to maximize the production of docosahexaenoic acid (DHA) and β-carotene by optimizing the culture conditions of their sources, microalgae Schizochytrium limacinum and fungus Blakeslea trispora respectively, in a fermentation medium. The influencing factors in the fermentation process for producing DHA and β-carotene have proven to be: the concentration of carbon source (different glycerol crude and pure concentrations) for both of them, and in particular temperature for DHA and pH for β-carotene. Testing the effect of these parameters was determined: biomass, DHA and β-carotene concentration. The highest production by S. limacinum was obtained at 25 °C, while using a quantity of 90 g/L of glycerol (crude or pure) as a carbon source. Temperature was the main factor that influenced the biosynthesis of DHA. The quantification of DHA was made by GC–MS chromatography, followed by a purification process, with the end result of DHA in pure phase. The maximum quantities for β-carotene production were obtained with pH 7 and 60 g/L of crude glycerol. The results highlight the possibility of using crude glycerol as a low-cost substrates for growth of microalgae S. limacinum and of fungus B. trispora in order to obtain the crucial molecules: docosahexaenoic acid and β-carotene.
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spelling pubmed-60040942018-06-26 Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora Bindea, Maria Rusu, Bogdan Rusu, Alexandru Trif, Monica Leopold, Loredana Florina Dulf, Francisc Vodnar, Dan Cristian Microb Cell Fact Review The goal of this research is the investigation of a way to maximize the production of docosahexaenoic acid (DHA) and β-carotene by optimizing the culture conditions of their sources, microalgae Schizochytrium limacinum and fungus Blakeslea trispora respectively, in a fermentation medium. The influencing factors in the fermentation process for producing DHA and β-carotene have proven to be: the concentration of carbon source (different glycerol crude and pure concentrations) for both of them, and in particular temperature for DHA and pH for β-carotene. Testing the effect of these parameters was determined: biomass, DHA and β-carotene concentration. The highest production by S. limacinum was obtained at 25 °C, while using a quantity of 90 g/L of glycerol (crude or pure) as a carbon source. Temperature was the main factor that influenced the biosynthesis of DHA. The quantification of DHA was made by GC–MS chromatography, followed by a purification process, with the end result of DHA in pure phase. The maximum quantities for β-carotene production were obtained with pH 7 and 60 g/L of crude glycerol. The results highlight the possibility of using crude glycerol as a low-cost substrates for growth of microalgae S. limacinum and of fungus B. trispora in order to obtain the crucial molecules: docosahexaenoic acid and β-carotene. BioMed Central 2018-06-16 /pmc/articles/PMC6004094/ /pubmed/29908562 http://dx.doi.org/10.1186/s12934-018-0945-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Bindea, Maria
Rusu, Bogdan
Rusu, Alexandru
Trif, Monica
Leopold, Loredana Florina
Dulf, Francisc
Vodnar, Dan Cristian
Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title_full Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title_fullStr Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title_full_unstemmed Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title_short Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora
title_sort valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using schizochytrium limacinum and blakeslea trispora
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004094/
https://www.ncbi.nlm.nih.gov/pubmed/29908562
http://dx.doi.org/10.1186/s12934-018-0945-4
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