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Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.

Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be sc...

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Autores principales: Luo, Lifei, Huang, Rong, Zhang, Aidi, Yang, Cheng, Chen, Liangming, Zhu, Denghui, Li, Yongming, He, Libo, Liao, Lanjie, Zhu, Zuoyan, Wang, Yaping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004361/
https://www.ncbi.nlm.nih.gov/pubmed/29623522
http://dx.doi.org/10.1007/s10142-018-0597-9
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author Luo, Lifei
Huang, Rong
Zhang, Aidi
Yang, Cheng
Chen, Liangming
Zhu, Denghui
Li, Yongming
He, Libo
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
author_facet Luo, Lifei
Huang, Rong
Zhang, Aidi
Yang, Cheng
Chen, Liangming
Zhu, Denghui
Li, Yongming
He, Libo
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
author_sort Luo, Lifei
collection PubMed
description Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10142-018-0597-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-60043612018-07-02 Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L. Luo, Lifei Huang, Rong Zhang, Aidi Yang, Cheng Chen, Liangming Zhu, Denghui Li, Yongming He, Libo Liao, Lanjie Zhu, Zuoyan Wang, Yaping Funct Integr Genomics Original Article Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10142-018-0597-9) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-04-05 2018 /pmc/articles/PMC6004361/ /pubmed/29623522 http://dx.doi.org/10.1007/s10142-018-0597-9 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Luo, Lifei
Huang, Rong
Zhang, Aidi
Yang, Cheng
Chen, Liangming
Zhu, Denghui
Li, Yongming
He, Libo
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title_full Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title_fullStr Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title_full_unstemmed Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title_short Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
title_sort selection of growth-related genes and dominant genotypes in transgenic yellow river carp cyprinus carpio l.
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004361/
https://www.ncbi.nlm.nih.gov/pubmed/29623522
http://dx.doi.org/10.1007/s10142-018-0597-9
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