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Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECR...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004453/ https://www.ncbi.nlm.nih.gov/pubmed/29844159 http://dx.doi.org/10.1073/pnas.1800592115 |
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author | Zhang, Xiaoyue Zhou, Wenkun Chen, Qian Fang, Mingming Zheng, Shuangshuang Scheres, Ben Li, Chuanyou |
author_facet | Zhang, Xiaoyue Zhou, Wenkun Chen, Qian Fang, Mingming Zheng, Shuangshuang Scheres, Ben Li, Chuanyou |
author_sort | Zhang, Xiaoyue |
collection | PubMed |
description | Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and asymmetrical divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during asymmetrical division of stem cells and ground tissue patterning. The Mediator subunit MED31 interacted with SCR but not SHR. Reduction of MED31 disrupted the spatiotemporal activation of CYCLIND6;1 (CYCD6;1), leading to defective asymmetrical division of stem cells generating ground tissue. MED31 was recruited to the promoter of CYCD6;1 in an SCR-dependent manner. MED31 was involved in the formation of a dynamic MED31/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of MED31 and SHR in different cell types regulates the dynamic formation of the ternary complex, which provides a tunable switch to strictly control the spatiotemporal transcriptional output. This study provides valuable clues to understand the mechanism by which master transcriptional regulators control organ patterning. |
format | Online Article Text |
id | pubmed-6004453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-60044532018-06-18 Mediator subunit MED31 is required for radial patterning of Arabidopsis roots Zhang, Xiaoyue Zhou, Wenkun Chen, Qian Fang, Mingming Zheng, Shuangshuang Scheres, Ben Li, Chuanyou Proc Natl Acad Sci U S A PNAS Plus Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and asymmetrical divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during asymmetrical division of stem cells and ground tissue patterning. The Mediator subunit MED31 interacted with SCR but not SHR. Reduction of MED31 disrupted the spatiotemporal activation of CYCLIND6;1 (CYCD6;1), leading to defective asymmetrical division of stem cells generating ground tissue. MED31 was recruited to the promoter of CYCD6;1 in an SCR-dependent manner. MED31 was involved in the formation of a dynamic MED31/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of MED31 and SHR in different cell types regulates the dynamic formation of the ternary complex, which provides a tunable switch to strictly control the spatiotemporal transcriptional output. This study provides valuable clues to understand the mechanism by which master transcriptional regulators control organ patterning. National Academy of Sciences 2018-06-12 2018-05-29 /pmc/articles/PMC6004453/ /pubmed/29844159 http://dx.doi.org/10.1073/pnas.1800592115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | PNAS Plus Zhang, Xiaoyue Zhou, Wenkun Chen, Qian Fang, Mingming Zheng, Shuangshuang Scheres, Ben Li, Chuanyou Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title | Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title_full | Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title_fullStr | Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title_full_unstemmed | Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title_short | Mediator subunit MED31 is required for radial patterning of Arabidopsis roots |
title_sort | mediator subunit med31 is required for radial patterning of arabidopsis roots |
topic | PNAS Plus |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004453/ https://www.ncbi.nlm.nih.gov/pubmed/29844159 http://dx.doi.org/10.1073/pnas.1800592115 |
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