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Mediator subunit MED31 is required for radial patterning of Arabidopsis roots

Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECR...

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Autores principales: Zhang, Xiaoyue, Zhou, Wenkun, Chen, Qian, Fang, Mingming, Zheng, Shuangshuang, Scheres, Ben, Li, Chuanyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004453/
https://www.ncbi.nlm.nih.gov/pubmed/29844159
http://dx.doi.org/10.1073/pnas.1800592115
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author Zhang, Xiaoyue
Zhou, Wenkun
Chen, Qian
Fang, Mingming
Zheng, Shuangshuang
Scheres, Ben
Li, Chuanyou
author_facet Zhang, Xiaoyue
Zhou, Wenkun
Chen, Qian
Fang, Mingming
Zheng, Shuangshuang
Scheres, Ben
Li, Chuanyou
author_sort Zhang, Xiaoyue
collection PubMed
description Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and asymmetrical divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during asymmetrical division of stem cells and ground tissue patterning. The Mediator subunit MED31 interacted with SCR but not SHR. Reduction of MED31 disrupted the spatiotemporal activation of CYCLIND6;1 (CYCD6;1), leading to defective asymmetrical division of stem cells generating ground tissue. MED31 was recruited to the promoter of CYCD6;1 in an SCR-dependent manner. MED31 was involved in the formation of a dynamic MED31/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of MED31 and SHR in different cell types regulates the dynamic formation of the ternary complex, which provides a tunable switch to strictly control the spatiotemporal transcriptional output. This study provides valuable clues to understand the mechanism by which master transcriptional regulators control organ patterning.
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spelling pubmed-60044532018-06-18 Mediator subunit MED31 is required for radial patterning of Arabidopsis roots Zhang, Xiaoyue Zhou, Wenkun Chen, Qian Fang, Mingming Zheng, Shuangshuang Scheres, Ben Li, Chuanyou Proc Natl Acad Sci U S A PNAS Plus Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and asymmetrical divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during asymmetrical division of stem cells and ground tissue patterning. The Mediator subunit MED31 interacted with SCR but not SHR. Reduction of MED31 disrupted the spatiotemporal activation of CYCLIND6;1 (CYCD6;1), leading to defective asymmetrical division of stem cells generating ground tissue. MED31 was recruited to the promoter of CYCD6;1 in an SCR-dependent manner. MED31 was involved in the formation of a dynamic MED31/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of MED31 and SHR in different cell types regulates the dynamic formation of the ternary complex, which provides a tunable switch to strictly control the spatiotemporal transcriptional output. This study provides valuable clues to understand the mechanism by which master transcriptional regulators control organ patterning. National Academy of Sciences 2018-06-12 2018-05-29 /pmc/articles/PMC6004453/ /pubmed/29844159 http://dx.doi.org/10.1073/pnas.1800592115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle PNAS Plus
Zhang, Xiaoyue
Zhou, Wenkun
Chen, Qian
Fang, Mingming
Zheng, Shuangshuang
Scheres, Ben
Li, Chuanyou
Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title_full Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title_fullStr Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title_full_unstemmed Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title_short Mediator subunit MED31 is required for radial patterning of Arabidopsis roots
title_sort mediator subunit med31 is required for radial patterning of arabidopsis roots
topic PNAS Plus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004453/
https://www.ncbi.nlm.nih.gov/pubmed/29844159
http://dx.doi.org/10.1073/pnas.1800592115
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